Combining Imaging with a Stimulant Delivery System to Study Live Mouse Sperm

October 13th, 2023

A Live Cell Imaging Technique to Study Calcium Signaling and Acrosome Exocytosis in Mouse Sperm (Video) | JoVE

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Collection and Washing of Sperm from the AcroSensE Mouse Model for Real-Time Imaging (Video) | JoVE

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Setting up the Single Cell Stimulant Delivery System for Real-Time Monitoring of Live Mouse Sperm (Video) | JoVE

01:19 min

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Combining Microscopy with a Stimulant Delivery System to Monitor Live Sperm from an AcroSensE Model Mouse (Video) | JoVE

01:45 min

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Roy Cohen^1,2, Danielle M. Sosnicki¹, Melissa A. White¹, Jacquelyn L. Nelson¹, Chinatsu Mukai¹, Alexander J. Travis^1,2

¹Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, ²Department of Public and Ecosystem Health, College of Veterinary Medicine, Cornell University

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מודל העכבר AcroSensE ושיטות הדמיה של תאים חיים המתוארים כאן מספקים גישה חדשה לחקר דינמיקת הסידן בתא התת-תאי של אקרוזום הזרע וכיצד הם מווסתים שלבי ביניים המובילים לאיחוי קרום ואקסוציטוזה אקרוזומית.

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Combining Imaging with a Stimulant Delivery System to Study Live Mouse Sperm

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