Name: resting membrane potential maintenance
Uploaded: 2023-04-30T10:11:17.000Z
Duration: 1 min 15 s
Channel: JoVE

A neurons&#39; resting membrane potential is maintained because of the differences in the ionic composition and permeability of two essential ions &#8212; sodium and potassium.
In the resting state, potassium ions move out through the potassium leak channels, creating an internal negative potential.
To balance the charges, sodium ions enter the cell through sodium leak channels and increase the membrane potential to negative 70 millivolts.
Thus, a cell at rest is like a leaky boat as the ions are being moved in and out of the cell. The sodium-potassium pump moves three sodium ions out and two potassium ions back into the cell to maintain the ionic gradient.
Suppose the sodium-potassium pump fails to function or is inhibited for more than 10 seconds.
The leak channels would continue to move ions until the equilibrium state is reached, where no further net movement of ions occurs.
So, the membrane potential falls or decays to zero in the absence of the gradient. With the loss of the membrane potential, the cell quickly loses its viability.

resting potential decay

Resting Potential Decay

The resting membrane potential of a neuron (-70mV) is sustained due to the selective ion permeability of the membrane. At the resting potential, the membrane is slightly permeable to ions like sodium (Na+) and chloride (Cl&#8722;) and highly permeable to potassium ions (K+). Differences in the ions&#39; concentration inside the cell compared to the outside are maintained by membrane transport proteins like channels and pumps.
At rest, the K+ is the main ion that moves across the membrane through potassium leak channels. K+ flowing out of the cell makes the cell interior more negative. Na+ moving slowly into the cell makes it slightly more positive than if only K+ movement were allowed. Hence, at the resting membrane potential, the cells&#39; negative interior is because of a much greater ability for K+ moving out than Na+ moving in. The driving force acting on the ions moves them down their electrochemical gradient. Though Cl&#8722; may not contribute significantly to the resting membrane potential, its permeability prevents abrupt changes in the membrane potential.
The efflux of K+ stops when the membrane potential reaches a value where this electrical driving force on K+ balances the effect of its concentration gradient. Therefore, to maintain the membrane potential, the cell expends energy to maintain the ionic concentrations. Therefore, the cell, which behaves like a leaky boat, allowing constant ionic movement, has the primary active transporter, the sodium-potassium (Na+/K+) pump. This pump moves Na+ out and K+ into the cell to counteract the constant movements of these ions down their electrochemical gradients. The pumps&#39; movement ensures that the ions&#39; concentration gradients are not dissipated, and the cell remains viable, even when not conducting a signal.
The Na+/K+ pump is not responsible for generating the membrane potential but for its maintenance, which it does by maintaining the normal intracellular K+ and Na+ concentrations. If the Na+/K+ pump fails to function, the entire ionic balance could be altered, sending the resting membrane potential to zero, detrimental to the cell. The resting potential is very crucial to the nervous system&#39;s functioning. The changes in membrane potential, such as the action potential, form the basis for neuronal signaling.

The resting membrane potential of a neuron (-70mV) is sustained due to the selective ion permeability of the membrane. At the resting potential, the ...

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JoVE Core

Cell Biology

Unit 1

Channels and the Electrical Properties of Membranes

channels-and-the-electrical-properties-of-membranes

KEY TERMS AND CONCEPTS

resting membrane potential maintenance

The resting membrane potential stays near -70 mV in a neuron because the membrane allows ions to move in a selective way. At rest, the membrane is slightly permeable to sodium ions (Na+) and chloride ions (Cl-) and highly permeable to potassium ions (K+). Ion channels and pumps keep the concentration differences between the inside and outside of the cell in place.

Potassium leak channels let K+ move out of the cell at rest. This outward movement makes the inside of the cell more negative. A small amount of Na+ also moves into the cell, which makes the membrane slightly more positive than it would be if only K+ were moving. The negative interior is mainly caused by the greater movement of K+ out of the cell than Na+ into the cell.

Ions move down their electrochemical gradient, which is the combined effect of concentration and electrical forces. Chloride ions do not add much to the resting membrane potential, but their permeability helps prevent sudden changes in membrane voltage. K+ stops leaving the cell when the electrical force balances its concentration gradient.

The cell must spend energy to keep these ion levels stable. The sodium-potassium pump, or Na+/K+ pump, moves Na+ out of the cell and K+ into the cell. This pump does not create the membrane potential, but it maintains it by preserving normal intracellular Na+ and K+ levels. The cell behaves like a leaky boat, with ions constantly moving through the membrane.

If the Na+/K+ pump fails, the ionic balance can change and the resting membrane potential can fall to zero. That would be harmful to the cell. The resting potential is essential for nervous system function, and changes in membrane potential, including the action potential, are the basis of neuronal signaling.

Resting Membrane Potential Maintenance

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The resting membrane potential stays near -70 mV in a neuron because the membrane allows ions to move in a selective way. At rest, the membrane is slightly permeable to sodium ions (Na+) and chloride ions (Cl-) and highly permeable to potassium ions (K+). Ion channels and pumps keep the concentration differences between the inside and outside of the cell in place. Potassium leak channels let K+ move out of the cell at rest. This outward movement makes the inside of the cell more negative. A...

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key terms and concepts

Learn how selective ion movement, leak channels, and the Na+/K+ pump maintain a neuron's resting membrane potential and support nerve signaling.

Resting Membrane Potential Maintenance | JoVE K12

cell biology

JoVE Core Cell Biology illustrates complex cellular processes in easy-to-understand yet comprehensive animated video lessons. This collection focuses on the essential components and functions of cells, including organelles and membranes, cellular transport, and cell energetics. It is complemented by scientist-in-action videos that demonstrate the application of cell biology in real-world research experiments.

Resting Membrane Potential Maintenance

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