Encyclopedia Of Experiments

Colorectal Cancer

Colorectal cancer originates within the colon or rectal regions of organisms. This collection highlights some fundamental research techniques in colorectal cancer relating to screening tests, genetic analysis, studies on the gut microbiome, therapeutic interventions, animal model generation, assay techniques, and cell behavior & metastatic responses.

Encyclopedia Of Experiments

Colorectal Cancer

Colorectal cancer originates within the colon or rectal regions of organisms. This collection highlights some fundamental research techniques in colorectal cancer relating to screening tests, genetic analysis, studies on the gut microbiome, therapeutic interventions, animal model generation, assay techniques, and cell behavior & metastatic responses.

Sections

Assays & techniques

Animal model studies

Assays & techniques

Animal model studies

Assays & techniques

Tumor Enzymatic Digestion

A Procedure to Generate Tumor Cell Suspension from Patient-derived Xenograft Cancer Mouse Models

Laser Microdissection

A Technique to Isolate Invading Colorectal Cancer cells from a 3D Organotypic Culture Model

Cytoskeleton and Focal Adhesion Organization Assay

An Immunofluorescence-based Method to Study Cell Adhesion and Spreading on Substrates

Spheroid Generation from Cell Lines

A Three-Dimensional (3D) Cell Culture Method

Microtubule Binding Assay in CRC Cells

A Method to Examine Microtubule Binding Tau Protein in Colorectal Cancer Cells

CRC Organoid Culture

A Method to Obtain 3D Organoids from Colorectal Cancer Cells

Traction Cytometry Assay

A Method to Quantify Traction Force Exerted by Cancer Cells on ECM

CTC Isolation from a Whole Blood Sample

A Method to Obtain CTCs from Murine Colorectal Cancer Model

CRC Organoid Cell Labeling

A Method to Generate GFP Lentivirus-transduced Colorectal Cancer Organoid Cells

Circulating miRNA Extraction

A Method to Isolate miRNA from Plasma Samples Via Organic Extraction and Small RNA Enrichment

ROS Detection with DCFH-DA Staining

Measuring Total ROS in Colorectal Cancer Cell Lines

Assessing DNA Damage Foci

To Identify Radiation-Induced DNA Damage Foci in Cancer Cell Line Using Immunofluorescence Staining

Stool Microbial DNA Isolation

Studying the Effect of Environmental Enrichment on Microbiome Diversity in Murine Tumor Model

Phosphatase Assay in CRC Cells

A Method to Examine the Phosphorylation Status of Tau Protein in Colorectal Cancer Cells

Animal model studies

Intracecal Tumor Cell Injection

A Technique to Deliver Cancer Cells for Establishing Orthotopic Colorectal Cancer Mouse Model

Segmental Adeno-Cre Infection

A Technique to Generate Isolated Colorectal Cancer using Genetically Engineered Mouse Models

Pretargeted Radioimmunotherapy

A Method for Targeted Delivery of Therapeutic Radionuclides to Tumors

Establishing Experimental Metastases Mouse Model

Implanting GFP Expressing CRC Organoid Cells in PDX Model to Detect Micrometastases

Spontaneous Metastasis Mouse Models

A Platform to Study the Metastatic Potential of Orthotopically Injected Colorectal Cancer Cells

Murine Ileal Resection Model

Studying Ileectomy-Induced Bile Metabolism in Murine Model

Generating PDOX Mouse Model of Colorectal Cancer

Studying Progression of Colorectal Cancer in a Murine Model