Encyclopedia Of Experiments

Head and Neck Cancer

Head and neck cancers constitute carcinomas originating in and around the mucosal surfaces of the head and neck regions. This collection features protocols relating to cancer modeling in vitro, in ovo, and in vivo, cell isolation and culture, fluorescent in situ hybridization-related techniques, generation of decellularized tissue matrices, and drug-resistance characterization assays.

Encyclopedia Of Experiments

Head and Neck Cancer

Head and neck cancers constitute carcinomas originating in and around the mucosal surfaces of the head and neck regions. This collection features protocols relating to cancer modeling in vitro, in ovo, and in vivo, cell isolation and culture, fluorescent in situ hybridization-related techniques, generation of decellularized tissue matrices, and drug-resistance characterization assays.

Sections

Assays & techniques

In vivo studies

Assays & techniques

In vivo studies

Assays & techniques

Magnetic Bead-based Exosome Extraction

A Technique to Isolate Specific Exosomes from Biofluids

Decellularized Porcine Esophageal Scaffold Preparation

A Technique to Generate Acellular Scaffold from a Pig Esophagus

Harvesting DRG from Rat Spine

A Procedure to Extract Dorsal Root Ganglion from the Spine of a Rat Model

Antibody Conjugated Nanoparticle-based Quantification Using Dark Field Microscopy

A Procedure to Capture and Quantify Specific Exosomes from Body Fluids

Establishing a Three-dimensional Co-culture Model of Murine Dorsal Root Ganglia and Cancer Cells

An In Vitro Model to Study Perineural Cancer Cell Invasion

Isolation and Culture of Murine Tongue Epithelial Cells

A Procedure to Isolate and Culture Tongue Epithelial Cells from Mouse Model

FFPE Tissue Pretreatment for RNA CISH

A Procedure to Process Formalin-Fixed Paraffin-Embedded Tissue Sections for RNA Chromogenic In Situ Hybridization

Probe Hybridization and Signal Amplification in RNA In Situ Hybridization

A Technique for Detecting Specific RNA Sequences in Tissue Sections

RNA CISH Signal Detection

A Technique to Detect Chromogenic Signals During RNA In Situ Hybridization in Intact Tissue Specimens

Fabrication of Extracellular Matrix from Mouse Model

An Effective Procedure to Prepare Decellularized Tongue Extracellular Matrix from Murine Tongue

Embedding 3D Spheroids in Agarose Gel Drops

A Technique to Preserve Spheroids for Downstream Analysis

Bioscaffold Fabrication by Electrospraying

A Technique to Generate Microcarrier Scaffolds Derived from Extracellular Matrix of Decellularized Tissue

Cancer Side Population Discrimination Assay

A Method to Isolate Cancer Stem-like Cells Using Hoechst Dye Efflux Assay

Cryomilling of Decellularized Tissue

A Technique to Obtain Fine Extracellular Matrix Powder

ECM Suspension Preparation

A Technique to Obtain Homogenous Suspension from Tissue-derived Extracellular Matrix

Extracellular Matrix-derived Foam Fabrication via Lyophilization

A Procedure to Generate Biological Scaffolds from ECM of Decellularized Tissues

Modeling Cancerous Neural Invasion In Vitro

A Method to Co-culture Dorsal Root Ganglia and Cancer Cells

Methyl Thiazolyl Tetrazolium or MTT Assay

A Colorimetric Assay to Measure Drug Resistance via Determination of Metabolic Activity in Cancer Cells

Sulforhodamine B Assay

A Sensitive Assay to Measure Drug Resistance Via Determination of Cellular Protein Content in Cancer Cells

Cell Death Screening Using DAPI Staining

A Method for Rapid Screening of IR Induced Clonogenic Cell Death in Cancerous Cell Lines

Esophageal 3D Raft Culture Model

An In Vitro Culture Technique to Generate Human Esophageal Mucosa Model

Culturing Salispheres from Salivary Gland Tissue

A Method to Develop Salispheres from Human Salivary Gland Derived Epithelial Cells

In vivo studies

Metastatic Cancer Cell Colony Isolation from Chicken CAM

A Procedure to Isolate Cancer Cells from Shell-less Egg Cultures

Orthotopic Inoculation of Thyroid Cancer Cells into Murine Thyroid

A Procedure to Establish Orthotopic Thyroid Cancer Mouse Model

Chick Chorioallantoic Membrane or CAM Tumor Model

An In Ovo Method to Simulate Perineural Invasion of Tumor Cells

Chick Chorioallantoic Membrane Harvest

A Method to Isolate Tumor Graft-bearing Chorioallantoic Membrane from Chicken Egg

Spinal Cord Extraction

A Protocol to Isolate Murine Spinal Cord for In Vitro Studies

Intravenous Injection of Cancer Cells into Chicken Chorioallantoic Membrane Vasculature

A Procedure to Establish Chorioallantoic Membrane Cancer Model to Study Cancer Invasion and Metastasis

Intramucosal Inoculation of Cancer Cells in Murine Model

A Procedure to Establish an Orthotopic Head and Neck Cancer Mouse Model

Modelling Intratumoral Treatment with miRNA Inhibitors

A Method to Analyze the Effect of miRNA Inhibitors on Tumor Growth in Murine Model