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Neutrophil Lifespan Extension with CLON-G and an In Vitro Spontaneous Death Assay
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JoVE Revista Biología
Neutrophil Lifespan Extension with CLON-G and an In Vitro Spontaneous Death Assay

Neutrophil Lifespan Extension with CLON-G and an In Vitro Spontaneous Death Assay

DOI:
10.3791/65132-v

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05:52 min

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May 12, 2023

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, , , , , ,
1State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital,Chinese Academy of Medical Sciences & Peking Union Medical College, 2Tianjin Institutes of Health Science, 3Sichuan Provincial People’s Hospital,University of Electronic Science and Technology of China, 4Haihe Laboratory of Cell Ecosystem,Tianjin Medical University, 5Joint Program in Transfusion Medicine, Department of Pathology, Harvard Medical School; Division of Blood Bank, Department of Laboratory Medicine, The Stem Cell Program, Boston Children’s Hospital,Dana-Farber /Harvard Cancer Center

Capítulos

  • 00:04Introduction
  • 00:44Neutrophil Lifespan Extension with CLON‐G
  • 02:13In Vitro Spontaneous Death Assay of Neutrophil
  • 04:06Results: Effect of CLON‐G Medium on Neutrophil Viability
  • 05:11Conclusion

Summary

Traducción Automática

Traducción Automática

This protocol details the preparation of CLON-G to extend the neutrophil lifespan to greater than 5 days and provides a reliable procedure for evaluating neutrophil death with flow cytometry and confocal fluorescence microscopy.

Tags

Neutrophil LifespanCLON-GIn Vitro Spontaneous Death AssayNeutrophil PreservationNeutrophil TransportationNeutrophil Gene ManipulationFlow CytometryFluorescent ImagingCell Culture

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