Name: nmr pulse angles and signal collection
Uploaded: 2024-04-04T10:51:41.000Z
Duration: 1 min 17 s
Channel: JoVE

パルスは、サンプル内の観測された核のラーマー周波数を含む高出力RF放射の短いバーストです。
原子核がエネルギーを吸収すると、正味の磁化ベクトルは横面に向かってシフトします。
磁化ベクトルのこの回転角度、つまり反転角度は、パルスの持続時間と強度に比例します。
90度のパルスは正味の磁化をx-y平面に正確にシフトし、180度のパルスはそれを-z方向にシフトします。
磁化がz軸に沿って平衡に戻ると、放出されたFID信号は集録時間中に収集されます。
パルスシーケンスは、送信機からの励起パルスで始まり、その後に集録時間が続きます。
その後、緩和遅延により、核が完全に弛緩し、次のパルスが適用される前に平衡を再確立することができます。
異なるNMR活性核を用いた実験では、マルチチャンネルパルスシーケンスを使用し、各チャンネルの周波数を1種類の核に一致させます。

nmr 分光計: 高周波パルスとパルス シーケンス

NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences

パルスは、サンプル内のすべての原子核を同時に励起する、ある範囲の周波数に分散された電波の短いバーストです。高周波パルスを x 軸に沿って通過させると、原子核はラーモア周波数に対応するエネルギーを吸収し、共鳴を達成します。これにより、正味の磁化ベクトルが z 軸から横断面に向かって移動します。磁化ベクトルの回転角、つまりフリップ角は、パルスの持続時間と強度に比例します。 90° パルスは正味の磁化を x-y 平面上に正確にシフトし、180° パルスはそれを -z 方向にシフトします。

パルスが連続して印加されることで、研究中のサンプルに関する詳細な情報が明らかになります。単純なパルス シーケンスは、送信機からの励起パルスで始まり、その後に自由誘導減衰信号が収集され、コンピューター コンソールによってデジタル化される取得時間が続きます。原子核は緩和し、次のパルスが続く緩和遅延時間に印加される前に平衡状態を再確立します。

NMR 分光計: 高周波パルスとパルス シーケンス

パルスは、サンプル内のすべての原子核を同時に励起する、さまざまな周波数に分布する電波の短いバーストです。通過すると...

nmr-spectrometers-radiofrequency-pulses-and-pulse-sequences

Education

JoVE Core

Analytical Chemistry

分析化学

Unit 1

ユニット1

Principles of Nuclear Magnetic Resonance

核磁気共鳴の原理

KEY TERMS AND CONCEPTS

主要な用語と概念

nmr pulse angles and signal collection

NMR pulse angles and signal collection explain how radiofrequency energy starts a measurement. A pulse is a short burst of radio waves spread across a range of frequencies. It excites the nuclei in the sample at the same time.

When a radiofrequency pulse is applied along the x-axis, nuclei absorb energy that matches their Larmor frequencies and reach resonance. The net magnetization vector moves away from the z-axis and toward the transverse plane. This rotation angle is called the flip angle. It depends on how long the pulse lasts and how strong it is.

A 90° pulse moves the net magnetization onto the x-y plane. A 180° pulse moves it to the -z direction. Pulses are then arranged in sequences to reveal more detail about the sample being studied.

A simple pulse sequence starts with an excitation pulse from the transmitter. It is followed by an acquisition time, when the free induction decay signals are collected and digitized by the computer console. The nuclei then relax and return toward equilibrium before the next pulse is applied during the relaxation delay.

NMR Pulse Angles and Signal Collection

nuclear magnetic resonance (nmr): overview

NMR Spectra and MRI Imaging

Nuclear magnetic resonance, or NMR, is a phenomenon that certain nuclei can show when they absorb radio frequency energy under the right conditions. Scientists use it in molecular spectroscopy and in medical imaging. In both cases, the sample or the person being studied is placed in a magnetic field and exposed to radio frequency energy. In NMR spectroscopy, the result is a spectrum. This spectrum plots the absorption frequencies of the sample against peak intensity. The pattern gives detailed...

atomic nuclei: nuclear spin

Nuclear Spin and NMR-Active Nuclei

Atomic nuclei have nuclear spin, which comes from the spins of their protons and neutrons. These particle spins are intrinsic angular momentum, and each electron, proton, and neutron has a spin value of 1/2. In nuclei, proton spins pair with other protons, and neutron spins pair with other neutrons. They do not pair with each other, so the final nuclear spin depends on which nucleons are left unpaired. Even numbers of protons do not add to the nuclear spin, and even numbers of neutrons do not...

atomic nuclei: nuclear magnetic moment

Nuclear Spin States and Magnetic Alignment

Atomic nuclei can act like tiny magnets when they have a nonzero spin. Because they are positively charged and rotating, they generate a magnetic field and a magnetic moment. In the absence of an external magnetic field, this magnetic moment points in random directions. When an external magnetic field, B0, is applied, the nuclear magnetic moments can line up with the field or against it. These two directions create 2I + 1 orientations. A hydrogen nucleus is a proton, and it has spin 1/2, so it...

atomic nuclei: nuclear spin state overview

atomic-nuclei-nuclear-spin-state-overview

Zeeman Splitting in Nuclear Spin States

NMR-active nuclei have spin states that describe the orientation of their nuclear magnetic moments. Without a magnetic field, these moments point in random directions, and the spin states are degenerate, meaning they have the same energy. When an external magnetic field is applied, the nucleus can only take on 2I + 1 orientations, where I is the nuclear spin. A proton with spin 1/2 has two possible orientations. A quadrupolar nucleus with spin 1 has three possible orientations. The...

atomic nuclei: nuclear spin state population distribution

atomic-nuclei-nuclear-spin-state-population-distribution

Nuclear Spin Populations and NMR Signal

Nuclear spin populations help explain why NMR signals appear. In a magnetic field, nuclei can occupy a lower energy spin-up state or a higher energy spin-down state. Near absolute zero, most nuclei stay in the lower energy state. As temperature rises, thermal collisions spread the spins more evenly between the two states. The balance between the spin −1/2 and spin +1/2 populations is described by the Boltzmann distribution equation. In this equation, ΔE is the energy difference, k is the...

atomic nuclei: larmor precession frequency

atomic-nuclei-larmor-precession-frequency

Magnetic Field and Nuclear Precession

Magnetic field strength controls nuclear precession in NMR. A spinning nucleus has a magnetic moment, and that moment turns around an external magnetic field directed along the z-axis. This motion is called Larmor precession, and its angular frequency is the Larmor frequency. The Larmor frequency is directly proportional to the applied magnetic field, B0. The proportionality constant is the gyromagnetic ratio, gamma. This value is fixed for each nucleus because every nucleus has its own ratio...

atomic nuclei: magnetic resonance

Nuclear Spins and Resonance in a Magnetic Field

Nuclear spins can line up in a magnetic field and create nuclear magnetic resonance. In the lower energy state, slightly more spins are aligned than in the higher energy state. That small difference gives the nuclei a net magnetization along the z axis.

atomic nuclei: nuclear relaxation processes

atomic-nuclei-nuclear-relaxation-processes

Nuclear Spin Alignment and Relaxation

Nuclear spin alignment and relaxation describe how nuclear spins respond to a magnetic field and then return to equilibrium. Without an external magnetic field, nuclear spin states are degenerate, meaning they have the same energy, and the spins are randomly oriented. When a magnetic field is applied, the spins begin to precess, or wobble, and orient themselves either along the field in a lower energy state or against it in a higher energy state. At equilibrium, a slight excess of spins...

atomic nuclei: types of nuclear relaxation

atomic-nuclei-types-of-nuclear-relaxation

NMR Relaxation Paths in Atomic Nuclei

Nuclear relaxation in atomic nuclei helps restore the balance between energy levels in NMR systems. It occurs through two main paths: spin-lattice relaxation and spin-spin relaxation. Both are first-order exponential decay processes. Spin-lattice relaxation is also called longitudinal relaxation. In this process, excited spins give energy to the surrounding lattice as they move back to the lower energy level. Magnetic dipolar interactions are an important part of this mechanism. A common...

nmr spectrometers: overview

How NMR Spectrometers Record Signals

NMR spectrometers record signals from samples that contain NMR-active nuclei. The system uses a strong magnet, a radiofrequency transmitter, and a detector connected to a computer console. These parts work together to measure and display the spectrum of the sample. Early NMR instruments were continuous-wave spectrometers. In a frequency-sweep method, the magnetic field stays fixed while the rf signal is swept. In a field-sweep method, the radiofrequency signal stays fixed while the magnetic...

nmr spectrometers: radiofrequency pulses and pulse sequences

NMR pulse angles and signal collection explain how radiofrequency energy starts a measurement. A pulse is a short burst of radio waves spread across a range of frequencies. It excites the nuclei in the sample at the same time. When a radiofrequency pulse is applied along the x-axis, nuclei absorb energy that matches their Larmor frequencies and reach resonance. The net magnetization vector moves away from the z-axis and toward the transverse plane. This rotation angle is called the flip angle.

nmr spectrometers: resolution and error correction

nmr-spectrometers-resolution-and-error-correction

NMR Peak Shape and Magnetic Field Shimming

NMR peak shape depends on the signal created after magnetic nuclei resonate and relax. The detected free induction decay is approximately exponential. When that decay is Fourier transformed, it becomes a Lorentzian peak in the frequency domain. A Lorentzian peak in an NMR spectrum is described by its amplitude, full width at half maximum, and position. Its width is governed by the spin-spin relaxation time alone. In real experiments, the sample and probe can make the magnetic field...

diamagnetic shielding of nuclei: local diamagnetic current

diamagnetic-shielding-of-nuclei-local-diamagnetic-current

How Electron Density Shields Nuclei in NMR

Electron density around a nucleus affects how strongly it is shielded in NMR spectroscopy. When an applied magnetic field acts on a molecule, the electrons begin to circulate. This creates a local diamagnetic current within the molecule. The circulating sigma-bonding electrons produce a local magnetic field, B local, that opposes the applied field, B0. The nucleus then feels an effective magnetic field, which is the difference between the applied field and the local field. This effect is...

key terms and concepts

Learn how NMR pulses excite nuclei, set flip angles, and collect free induction decay signals during a simple pulse sequence in the spectrometer.

NMR Pulse Angles and Signal Collection | JoVE K12

JoVE Core 分析化学 は、複雑な化学原理をアニメーションで視覚化することで、SI 単位から NMR 分光法を含むトピックをよりわかりやすくします。実験を撮影した実写動画では、これらの概念が世界中の研究所でどのように応用されているかを取り上げています。

analytical chemistry

NMR Pulse Angles and Signal Collection

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