JOVE JOURNAL

Immunology and Infection

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Journal (Immunology and Infection)

Procedures for In Vitro Cultivation of Treponema pallidum, the Syphilis Spirochete

Diane G. Edmondson¹, Steven J. Norris¹, Bridget D. De Lay¹

¹University of Texas Health Science Center at Houston - McGovern Medical School

Journal (Immunology and Infection)

Assay Development for High-Throughput Drug Screening Against Mycobacteria

Gabriel S. Oliveira^1,2, Clara M. Bento^1,3,4, António Pombinho^1,3, Rita Reis^1,3, Kevin Van Calster^5,6, Linda De Vooght⁵, André F. Maia^1,3, Paul Cos⁵, Maria Salomé Gomes^1,2, Tânia Silva^1,2

¹i3S – Instituto de Investigação e Inovação em Saúde da Universidade do Porto, ²ICBAS – Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, ³IBMC - Instituto de Biologia Molecular e Celular, Universidade do Porto, ⁴Programa Doutoral em Biologia Molecular e Celular (MCBiology),Instituto de Ciências Biomédicas Abel Salazar da Universidade do Porto, ⁵Laboratory for Microbiology,Parasitology and Hygiene (LMPH), ⁶Department of Infectious Diseases in Humans, Immune Response,Sciensano

Journal (Immunology and Infection)

In Vitro Differentiation of Naive CD4⁺ T Cells into Pathogenic Th17 Cells in Mouse

Haofei Wang*¹, Xu Liu*¹, Wei Huang¹, Xiaojing Wu¹, Ying Tang¹, Mingzhu Zheng^1,2, Haibo Qiu¹, Yingzi Huang¹

¹Jiangsu Provincial Key Laboratory of Critical Care Medicine, Department of Critical Care Medicine, Zhongda Hospital, School of Medicine,Southeast University, ²Department of Pathogenic Biology and Immunology, Jiangsu Provincial Key Laboratory of Critical Care Medicine, School of Medicine,Southeast University

Imaging Initial Ca2+ Microdomains in Primary T Cells

Journal (Immunology and Infection)

Imaging Initial Ca²⁺ Microdomains in Primary T Cells

Fynn Gerlach*¹, Franziska Möckl*¹, Dejan Kovacevic^1,2,3, Valerie J. Brock¹, Riekje Winzer⁴, Lina Meyer^2,3, David Lohr^2,3, Lena-Marie Woelk^2,3, Eva Tolosa⁴, René Werner^2,3, Björn-Philipp Diercks¹

¹The Calcium Signalling Group, Department of Biochemistry and Molecular Cell Biology,University Medical Centre Hamburg-Eppendorf, ²Department of Applied Medical Informatics,University Medical Centre Hamburg-Eppendorf, ³Department of Computational Neuroscience,University Medical Centre Hamburg-Eppendorf, ⁴Department of Immunology,University Medical Centre Hamburg-Eppendorf

Journal (Immunology and Infection)

Whole Blood Assay with Dual Co-Stimulation for Antigen-Specific Analysis of Host Immunity to Fungal and Viral Pathogens

Lukas Page¹, Chris D. Lauruschkat², Kevin Dennehy¹, Eva Loell¹, Beeke Tappe², Andre Fuchs³, Sebastian Wurster*⁴, Reinhard Hoffmann*¹

¹Institute for Laboratory Medicine and Microbiology,University Hospital Augsburg, ²Department of Internal Medicine II,University Hospital of Wuerzburg, ³Internal Medicine III - Gastroenterology and Infectious Diseases,University Hospital Augsburg, ⁴Department of Infectious Diseases, Infection Control and Employee Health, MD Anderson Cancer Center,University of Texas Houston

Journal (Immunology and Infection)

Engineering of Human Blood-Induced Microglia-like Cells for Reverse-Translational Brain Research

Sota Kyuragi*¹, Shogo Inamine*¹, Masahiro Ohgidani^1,2, Takahiro A. Kato¹

¹Department of Neuropsychiatry, Graduate School of Medical Sciences,Kyushu University, ²Department of Functional Anatomy and Neuroscience,Asahikawa Medical University

Journal (Immunology and Infection)

Identification of Rare Antigen-Specific T Cells from Mouse Lungs with Peptide:Major Histocompatibility Complex Tetramers

Daniel S. Shin^1,2,3, Juliana Barreto de Albuquerque^1,3, James J. Moon^1,3,4

¹Center for Immunology and Inflammatory Diseases, Division of Rheumatology, Allergy, and Immunology,Massachusetts General Hospital, ²Division of Immunology,Boston Children's Hospital, ³Harvard Medical School, ⁴Division of Pulmonary and Critical Care Medicine,Massachusetts General Hospital

Journal (Immunology and Infection)

Electronic von Frey associated with the Mouse Grimace Scale to Assess Allodynia and Pain in Trypanosoma evansi-Infected Mice

Daniel Sérgio Cipriani¹, Gabriela Kaiser Borges², Luiz Claudio Miletti², Amanda Leite Bastos-Pereira^2,3

¹Residency Program in Veterinary Medicine,Santa Catarina State University, ²Multicenter Postgraduate Program in Biochemistry and Molecular Biology,Santa Catarina State University, ³Federal University of Santa Catarina

Journal (Immunology and Infection)

Sequential Blood Collection from Inferior Vena Cava Followed by Portal Vein to Evaluate Gut Microbial Metabolites in Mice

Thien N. M. Doan¹, Daisuke Maruyama¹, Xiaoli Tian¹, Arun Prakash¹

¹Department of Anesthesia and Perioperative Care,University of California San Francisco and San Francisco General Hospital

Journal (Immunology and Infection)

Measuring Endoplasmic Reticulum Stress and Unfolded Protein Response in HIV-1 Infected T-Cells and Analyzing its Role in HIV-1 Replication

Anjali Tripathi¹, Anindita Dasgupta¹, Debashis Mitra¹

¹National Centre for Cell Science,SP Pune University Campus

Journal (Immunology and Infection)

Examination of Pyroptosis by Flow Cytometry

He Chang^1,2,3, Zhi Chen^1,2,3, Li Gao^1,2,3, Hong Cao^1,2,3, Yongqiang Wang^1,2,3, Shijun J. Zheng^1,2,3

¹National Key Laboratory of Veterinary Public Health Security, ²Animal Epidemiology of the Ministry of Agriculture, ³College of Veterinary Medicine,China Agricultural University

Journal (Immunology and Infection)

Demystifying In Vivo Bioluminescence Imaging of a Chagas Disease Mouse Model for Drug Efficacy Studies

Adriana C. da Silva¹, Jadel M. Kratz², Priscylla G. M. Morgado^1,3, Lucio H. Freitas-Junior¹, Carolina B. Moraes^1,4

¹Departamento de Microbiologia, Instituto de Ciências Biomédicas (ICB),Universidade de São Paulo, ²Drugs for Neglected Diseases initiative (DNDi) Latin America, ³Programa de Pós-Graduação em Microbiologia e Imunologia,Universidade Federal de São Paulo, ⁴Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas,Universidade de São Paulo

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