Antibiotic Resistance in Cloning

Antibiotic resistance genes help scientists find bacteria that took up a plasmid with a gene of interest. In genetic engineering, this resistance is used as a marker, not just as a medical concern. The same process also connects to the growing problem of resistant bacteria in the real world.

Anti...

Antibiotic selection is typically used to ensure that a bacterial colony contains a plasmid with a few key features, an antibiotic resistance gene and a gene of interest that a researcher wants to express.

For the bacteria to uptake the plasmid, both must be incubated together and then, undergo heat shock to promote incorporation. Not ev...