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Semi-Automated Mechanical-Enzymatic Tissue Dissociation for Single-Cell Analysis

November 10th, 2023
1, 2, 2, 2, 2, 1, 1, 1
1Fischell Department of Bioengineering, University of Maryland, 2UMD Terrapin Works, University of Maryland

Summary
Automatic Translation

A general protocol for the combined enzymatic and semi-automated mechanical dissociation of tissues to generate single-cell suspensions for downstream analyses, such as flow cytometry, is provided. Instructions for the fabrication, assembly, and operation of the low-cost mechanical device developed for this protocol are included.

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