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DREADDs Technology and Automated Blood Sampling

August 25th, 2023
1, 1,2, 1,2, 1,2
1Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, 2Mouse Metabolic Phenotyping Center-Live, University of Michigan, Ann Arbor

Summary
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Luteinizing hormone (LH) pulsatility is a hallmark of reproductive function. We describe a protocol for remote activation of specific neuronal populations linked to serial automated blood collection. This technique allows timed hormonal modulation, multiplexing, and minimizing manipulation effects on LH levels in conscious freely moving, and undisturbed animals.

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