Hubei University of Medicine 1 article published in JoVE Medicine Isolation and Identification of Limbal Niche Cells Guanyu Su1, Wei Wang1, Lingjuan Xu1, Rong Liu1, Yongyao Tan1, Bihui Jin1, Chunxiu You1, Xiao Zhou1, Yihong Xiong2, Huatao Xie3, Guigang Li1 1Department of Ophthalmology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 2Institute of Medicine Nursing, Hubei University of Medicine, 3Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology Here, we present a protocol to isolate and identify the human limbal niche cells.