Osaka University Graduate School of Dentistry 1 article published in JoVE Developmental Biology In Vitro Investigation of the Effects of the Hyaluronan-Rich Extracellular Matrix on Neural Crest Cell Migration Toshihiro Inubushi1 1Department of Orthodontics and Dentofacial Orthopedics, Osaka University Graduate School of Dentistry This protocol outlines an in vitro migration experiment suitable for the functional analysis of the molecules involved in the in vivo migration of neural crest cells into the hyaluronan-rich extracellular matrix.