University of Nottingham Malaysia Campus 1 article published in JoVE Genetics Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX Mohamed I. Hassan*1, Fern R. McSorley*1, Kinya Hotta2, Christopher N. Boddy1 1Department of Chemistry and Biomolecular Sciences, University of Ottawa, 2School of Biosciences, The University of Nottingham Malaysia Campus This study describes methods for the T7-mediated co-expression of multiple genes from a single plasmid in Escherichia coli using the pMGX plasmid system.