Heart Research Center Goettingen 1 article published in JoVE Bioengineering Analysis of Tubular Membrane Networks in Cardiac Myocytes from Atria and Ventricles Eva Wagner*1,2,3, Sören Brandenburg*1,2, Tobias Kohl1,2, Stephan E. Lehnart1,2,3,4 1Heart Research Center Goettingen, 2Clinic of Cardiology & Pulmonology, University Medical Center Goettingen, 3German Center for Cardiovascular Research (DZHK) partner site Goettingen, 4BioMET, Center for Biomedical Engineering & Technology, University of Maryland School of Medicine In cardiac myocytes, tubular membrane structures form intracellular networks. We describe optimized protocols for i) isolation of myocytes from mouse heart including quality control, ii) live cell staining for state-of-the-art fluorescence microscopy, and iii) direct image analysis to quantify the component complexity and the plasticity of intracellular membrane networks.