Amgen, Inc. 4 articles published in JoVE Medicine Isolation and Purification of Murine Cardiac Pericytes Linda L. Lee1, Aarif Y. Khakoo2, Vishnu Chintalgattu1 1Department of Cardiometabolic Disorders, Amgen Research and Discovery, Amgen Inc., 2Department of Translational Sciences, Amgen Research and Discovery, Amgen Inc. We have optimized a protocol to isolate and purify murine cardiac pericytes for basic research and investigation of their biology and therapeutic potential. Genetics In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression Nicole A. Vander Schaaf1, Shirley Oghamian2, Jin-A Park1, Liang Kang1, Peter W. Laird1, Kwang-Ho Lee1 1Center for Epigenetics, Van Andel Research Institute, 2Amgen This protocol outlines the steps needed to generate a model system in which the transcription of an endogenous gene of interest can be conditionally controlled in live animals or cells using enhanced lac repressor and/or tet activator systems. Chemistry Application and Methodology of the Non-destructive 19F Time-domain NMR Technique to Measure the Content in Fluorine-containing Drug Products Maria Victoria Silva Elipe1, Lan Li1, Karthik Nagapudi2, Alan M. Kook3, Carlos Cobas4, Isaac Iglesias4, Chen Peng4 1Department of Attribute Sciences, Amgen, Inc., 2Small Molecule Pharmaceutical Sciences, Genentech, Inc., 3NMR Service + Consulting, 4Mestrelab Research A simple and non-destructive technique that measures the average content of drug substances in formulated drug products containing fluorine using low-field fluorine-19 (19F) time-domain (TD) nuclear magnetic resonance (NMR) is presented here. The technique can be applied to the development and manufacturing of drugs in the pharmaceutical industry. Medicine Dry Powder and Nebulized Aerosol Inhalation of Pharmaceuticals Delivered to Mice Using a Nose-only Exposure System Jonathan E. Phillips1, Xuxia Zhang1, James A. Johnston1 1Inflammation Research, Amgen The inhalation unit described herein can generate, sample for characterization, and uniformly deposit a drug aerosol in the lungs of rodents. This enables the pre-clinical determination of the efficacy and safety of drug doses deposited in the lungs; key data enabling clinical inhaled drug development.