Reed Mariculture 1 article published in JoVE Developmental Biology The Complete and Updated "Rotifer Polyculture Method" for Rearing First Feeding Zebrafish Christian Lawrence1, Jason Best1, Jason Cockington2, Eric C. Henry3, Shane Hurley1, Althea James1, Christopher Lapointe1, Kara Maloney1, Erik Sanders4 1 Larval zebrafish are adapted to feed on zooplankton. It is possible to capitalize on this natural feature in the laboratory by growing first feeding fish together in the same system with live saltwater rotifers. This "polyculture" strategy promotes high growth and survival with minimal labor and disturbance to the larvae.