Protocol
Preparation, Imaging, and Quantification of Bacterial Surface Motility Assays
Nydia Morales-Soto1,2, Morgen E. Anyan1, Anne E. Mattingly1, Chinedu S. Madukoma1, Cameron W. Harvey3, Alber Mark3, Eric Déziel4, Daniel B. Kearns5, Joshua D. Shrout1,2,6
1Department of Civil and Environmental Engineering and Earth Sciences, University of Notre Dame, 2Eck Institute for Global Health, University of Notre Dame, 3Department of Applied and Computational Mathematics and Statistics, University of Notre Dame, 4INRS-Institut Armand-Frappier, 5Department of Biology, Indiana University, 6Department of Biological Sciences, University of Notre Dame
Swarming motility is influenced by physical and environmental factors. We describe a two-phase protocol and guidelines to circumvent the challenges commonly associated with swarm assay preparation and data collection. A macroscopic imaging technique is employed to obtain detailed information on swarm behavior that is not provided by current analysis techniques.
The Modified Hole Board – Measuring Behavior, Cognition and Social Interaction in Mice and Rats
Maaike Labots1,2, Hein A. Van Lith1,2, Frauke Ohl1,2, Saskia S. Arndt1,2
1Department of Animals in Science and Society, Utrecht University, 2Brain Center Rudolf Magnus
This protocol describes the modified hole board, which is a behavioral test set-up that comprises the characteristics of an open field and a traditional hole board. This set-up enables the differential analysis of unconditioned behavior of small laboratory mammals as well as the analysis of cognitive abilities.
Functional Human Liver Preservation and Recovery by Means of Subnormothermic Machine Perfusion
Bote G. Bruinsma*1, James H. Avruch*2, Pepijn D. Weeder1, Gautham V. Sridharan1, Basak E. Uygun1, Negin G. Karimian1, Robert J. Porte3, James F. Markmann2, Heidi G. Yeh2, Korkut Uygun1
1Center for Engineering in Medicine, Dept. of Surgery, Massachusetts General Hospital, Harvard Medical School, 2Transplant Center, Dept. of Surgery, Massachusetts General Hospital, Harvard Medical School, 3Section of Hepatobiliary Surgery and Liver Transplantation, Department of Surgery, University Medical Center Groningen, University of Groningen
We describe a method of ex vivo machine perfusion of human liver grafts at subnormothermic temperature (21°C).
An Assay for Measuring the Effects of Ethanol on the Locomotion Speed of Caenorhabditis elegans
Andrew G. Davies1,2, GinaMari G. Blackwell1,2, Richard C. Raabe1, Jill C. Bettinger1,2
1Department of Pharmacology and Toxicology, Virginia Commonwealth University, 2VCU Alcohol Research Center, Virginia Commonwealth University
C. elegans is a useful model for studying the effects of ethanol on behavior. We present a behavioral assay that quantifies the effects of ethanol on the locomotion speed of crawling worms; both initial sensitivity and the development of acute functional tolerance to ethanol can be measured with this assay.
Disclosures
No conflicts of interest declared.