Name: dna and rna in cells
Uploaded: 2023-04-30T18:28:47.000Z
Duration: 2 min 43 s
Channel: JoVE

K12 Standards

Next Generation Science Standards

High School

Life Science

from-molecules-to-organisms-structures-and-processes

From Molecules to Organisms: Structures and Processes

HS-LS1-1

Nucleic Acids

Нуклеиновые кислоты полимеры нуклеотидов 13 молекул, состоящих из пентозного сахара, азотсодержащего основания и фосфатной группы. Есть два типа нуклеиновых кислот:дезоксирибонуклеиновая кислота и рибонуклеиновая кислота Их химическая структура различается в зависимости от того, какие пентозные сахара и азотистые основания они содержат. Пентозный сахар в РНК это рибоза, у которой есть гидроксильная группа, присоединенная к углероду-2.Сахар в ДНК это дезоксирибоза, которая имеет только атом водорода, но не имеет кислорода при углероде-2. Азотистое основание связано с углеродом-1, а фосфат с углеродом-5. И РНК, и ДНК содержат основания аденин, цитозин гуанин, но в ДНК есть тимин, а в РНК урацил.В ДНК и РНК гуанин и цитозин образуют комплементарные пары оснований, связанные тремя водородными связями. Аденин и тимин образуют пары оснований в ДНК, в то время как аденин и урацил образуют пары в РНК, оба связаны друг с другом двумя водородными связями. Различные ферменты ДНК или РНК-полимеразы катализируют полимеризацию нуклеотидов.Фосфодиэфирная связь образуется между гидроксильной группой, присоединенной к углероду-3, и фосфатной группой, присоединенной к углероду-5 следующего нуклеотида. Эта реакция оставляет неприсоединенный конец 5'со свободной фосфатной группой и неприсоединенный конец 3'со свободной гидроксильной группой. При соединении с комплементарной нитью две молекулы антипараллельны, то есть конец 5'одной нити соединяется с концом 3'другой.Нити удерживаются вместе межмолекулярными силами, включая гидрофобные эффекты, ван-дер-ваальсовы взаимодействия и специфические водородные связи, которые образуются между азотистыми основаниями. ДНК представляет собой двойную спираль, состоящую из двух полинуклеотидных цепей, намотанных друг на друга. Напротив, РНК часто встречается в виде одноцепочечной молекулы.Однако РНК может связываться с комплементарной РНК или ДНК. Он также может демонстрировать внутрицепочечные комплементарные пары оснований, приводящие к различным типам вторичных структур РНК, которые выполняют различные функции внутри клетки.

Нуклеиновые кислоты

Нуклеиновые кислоты являются наиболее важными макромолекулами для непрерывности жизни. Они несут генетический план клетки и несут инструкции по ее функционированию. 
 ДНК и РНК 
 Двумя основными типами нуклеиновых кислот являются дезоксирибонуклеиновая кислота (ДНК) и рибонуклеиновая кислота (РНК). ДНК является генетическим материалом для всех живых организмов, начиная от одноклеточных бактерий и заканчивая многоклеточными млекопитающими. Он находится в ядре эукариот и в оргалах, хлоропластах и митохондрии. У прокариот ДНК не заключена в мембранозный конверт. 
 Генетическое содержание клетки – это ее геном, а исследование геномов – геномика. В эукариотических клетках, но не в прокариотах, ДНК образует комплекс с протеинами гистона, образующими хроматин, вещество эукариотических хромосом. Хромосома может содержать десятки тысяч генов. Многие гены содержат информацию о том, как создавать белки. Другие гены кода продуктов РНК. ДНК контролирует все клеточные действия, включая или выключая гены. 
 Другой тип нуклеиновой кислоты, РНК, в основном участвует в синтезе белков. Молекулы ДНК никогда не покидают ядро, а вместо этого используют посредника для связи с остальными элементами клетки. Этот посредник — мессенджер РНК (мРНК). Другие типы РНК, такие как рРНК, тРНК и микроРНК, участвуют в синтезе белков и его регулировании. 
 ДНК и РНК состоят из мономеров, называемых нуклеотидными. Каждый нуклеотид состоит из трех компонентов: Нитрогенозный основание, пентозный (пятиугольный) сахар и группа фосфатов. Каждый нитрогенизный основание в нуклеотидном приливе прикрепляется к молекуле сахара, которая присоединена к одной или нескольким фосфатным группам. Нитрогеновые основания, важные компоненты нуклеотидов, являются органическими молекулами и называются так, потому что они содержат углерод и азот. Они являются основаниями, поскольку содержат аминогруппу, которая имеет потенциал связывания дополнительного водорода, и, таким образом, уменьшает концентрацию ионов водорода в окружающей среде, делая ее более базовой. Каждый нуклеотид в ДНК содержит одну из четырех возможных нитрогенозных основ: Аденин (A), гуанин (G), цитозин (C) и тимин (T). Аденин и гуанин классифицируются как пурины. Основная структура пурина состоит из двух колец с углеродным азотом. Цитозин, тимин и урацил классифицируются как пиримидин, которые имеют одно кольцо углерода-азота в качестве своей основной структуры. К каждому из этих базовых колец с углеродным азотом присоединены различные функциональные группы. В сокращениях молекулярной биологии мы знаем нитрогеновые основы по их символам A, T, G, C и U. ДНК содержит A, T, G и C; тогда как, РНК содержит A, U, G и C. 
 Пентозный сахар в ДНК дезоксирибозе, а в РНК сахар рибозе. Разница между сахарами заключается в наличии гидроксильной группы на втором углероде рибозы и водороде на втором углероде дезоксирибозы. Осадок фосфатов прикрепляется к гидроксильной группе углерода 5′ одного сахара и гидроксильной группе углерода 3′ сахара следующего нуклеотида, образующей связь фосфодиэфира 5′–3′. 
 Структура ДНАА Double-Helix 
 ДНК имеет двуспиральный состав. Сахар и фосфат лежат на внешней стороне геликоида, образуя костяк ДНК. В интерьере сложены нитрогенные основания, как пара лестничных ступеней. Водородные связи связывают пары друг с другом. Каждая пара основание в двойной спирали отделена от следующей пары основание на 0.34 нм. Две нити геликоида работают в противоположных направлениях, что означает, что угольный конец одной нити 5′ будет направлен в сторону углеродного конца соответствующей нити 3′. Допускается только некоторые типы сопряжения основание - СОЕДИНЕНИЕ CAN с T, и G может соединиться с C. это дополнительное правило основание. Другими словами, нити ДНК взаимно дополняют друг друга. 
 РНК 
 Рибонуклеиновая кислота, или РНК, в основном участвует в процессе синтеза белков под направлением ДНК. РНК обычно одноцепочечные и состоят из рибонуклеотидов, связанных фосфодиестными связями. 
 Существует четыре основных типа РНК: messenger RNA (mRNA), рибосомная РНК (rRNA), Transfer RNA (tRNA), и microRNA (miRNA). Первая, mRNA, передает сообщение от ДНК, которая контролирует все клеточные активности в клетке. Если клетке требуется определенный белок, то ген для него включается, и в ядре синтезируется посыльный РНК. Последовательность RNA основание дополняет последовательность кодирования ДНК, из которой она была скопирована. В цитоплазме мРНК взаимодействует с рибосомами и другими клеточными механизмами. 
 МРНК читается в наборах из трёх оснований, известных как кодоны. Каждый кодон кодирует одну аминокислоту. Таким образом, мРНК считывается и производится белковый продукт. Рибосомальная РНК (rRNA) является основным компонентом рибосом, на котором связывается мРНК. РНК обеспечивает надлежащее выравнивание мРНК и рибосом. РНК рибосомы также обладает энзиматической активностью (пептидилтрансферазы) и катализирует образование пептидной связи между двумя выровненными аминокислотами. РНК переноса (tRNA) — один из самых маленьких из четырех типов РНК, обычно 70–90 нуклеотидов длиной. Он переносит правильную аминокислоту на место синтеза белка. Это соединение основание между тРНК и мРНК, которое позволяет правильной аминокислоте вставлять себя в полипептидную цепь. МикроРНК являются самыми маленькими молекулами РНК, и их роль включает в себя регулирование экспрессии генов путем вмешательства в экспрессию определенных сообщений мРНК. 
 Несмотря на то, что РНК одноцепочечные, большинство типов РНК показывают обширное внутримолекулярное сопряжение основание между комплементарными последовательностями, создавая предсказуемую трехмерную структуру, необходимую для их функции. 
 Этот текст адаптирован из <a href="https://openstax.org/books/biology-2e/pages/3-5-nucleic-acids"> Openstax, Biology 2e, Глава 3.5: Нуклеиновые кислоты</a>.

Нуклеиновые кислоты являются наиболее важными макромолекулами для продолжения жизни. Они несут генетический чертеж клетки и инструкции по ее ...

Education

JoVE Core

Cell Biology

Клеточная биология

Unit 1

Раздел 1

Biochemistry of the Cell

Биохимия клетки

KEY TERMS AND CONCEPTS

ОСНОВНЫЕ ТЕРМИНЫ И ПОНЯТИЯ

dna and rna in cells

DNA and RNA are nucleic acids that help store and use genetic information in cells. They are key molecules in biology and are often studied together because they work as a pair in living systems.

Nucleic acids are built from repeating units called nucleotides. Each nucleotide has a sugar, a phosphate group, and a nitrogenous base. The order of these bases carries the genetic code.

There are two main nucleic acids: DNA, or deoxyribonucleic acid, and RNA, or ribonucleic acid. DNA usually stores genetic information, while RNA helps carry and use that information in the cell. Their roles make them essential for heredity and cell function.

DNA and RNA in Cells

the periodic table and organismal elements

the-periodic-table-and-organismal-elements

Elements in the Human Body and Periodic Table

Elements are the smallest units of matter that cannot be broken down further by chemical processes. Scientists know 118 elements, but not all of them occur naturally. Only a small number are essential for life, and living matter depends mostly on carbon, nitrogen, hydrogen, and oxygen. The periodic table organizes elements by their physical and chemical properties. Each square shows the element name, chemical symbol, atomic number, and atomic weight. The atomic number is the number of protons...

functional groups

Functional Groups in Organic Molecules

Functional groups are the part of an organic molecule that gives it specific chemical behavior. They help explain why compounds with similar carbon skeletons can react in different ways. In organic chemistry, these groups are often used to classify compounds. A functional group can change the properties of a molecule and affect how it behaves in a reaction. Knowing functional groups helps students recognize patterns in organic compounds. It also makes it easier to compare molecules that share...

types of chemical bonds

Chemical Bonds and How Atoms Join

Chemical bonds hold atoms together and help explain how different substances form. In chemistry, the main bond types are ionic bonds, covalent bonds, and metallic bonds. Ionic bonds form when electrons move from one atom to another. Covalent bonds form when atoms share electrons. Metallic bonds occur in metals, where electrons are held in a shared arrangement that gives metals their characteristic behavior. Understanding these bond types helps students compare how atoms connect in different...

noncovalent attractions in biomolecules

Biomolecule Interactions Without Covalent Bonds

Biomolecules often stay together through noncovalent attractions instead of covalent bonds. These weaker interactions help molecules recognize each other and form structures needed for life. The main noncovalent attractions are hydrogen bonds, electrostatic interactions, van der Waals forces, and hydrophobic interactions. Each one works in a different way, but all of them can influence how biomolecules fold, bind, and behave in cells. Hydrogen bonds form when a hydrogen atom is attracted to...

polymers

Polymer Chains and Their Properties

Polymers are large molecules made from repeating units called monomers. These long chains can form many common materials. Their structure helps explain why different polymers can behave in different ways. Polymers can be built in more than one way. Some form by addition polymerization, where monomers join together directly. Others form by condensation polymerization, which releases a small molecule as the chain grows. The type of polymer affects its properties and uses. Some polymers are...

what are lipids?

Lipids in Cells: Membranes, Energy, and Signals

Lipids are important cell molecules that act as membrane parts, energy stores, and signaling molecules. They are a diverse group of hydrocarbons, which are compounds made of carbon and hydrogen atoms. The carbon-carbon and carbon-hydrogen bonds are nonpolar, so lipids have an overall nonpolar character. Lipids are also hydrophobic, or “water-hating.” They do not form hydrogen bonds with water, so they are nearly insoluble in water. They are, however, soluble in organic solvents such as...

structure of lipids

How Lipid Molecules Are Built

Lipid molecules are built from hydrocarbon chains and a few key structural parts. Their shape helps explain why they behave differently from many other biological molecules. In high school biology, this structure is often linked to how lipids store energy and help form cell membranes. Some lipids are made from fatty acids joined to other small molecules. Triglycerides contain glycerol and three fatty acids, while phospholipids contain glycerol, two fatty acids, and a phosphate group. These...

chemistry of carbohydrates

Carbohydrate Structure and Bonding

Carbohydrates are built from sugar units and the bonds that link them. Their chemistry explains how simple sugars join to form larger molecules. It also helps show why carbohydrates have different structures and functions. The video focuses on the main sugar types found in carbohydrates. These include monosaccharides, disaccharides, and polysaccharides. Monosaccharides are single sugar units. Disaccharides contain two sugar units, while polysaccharides are long chains of many sugar units. The...

nucleic acids

DNA and RNA are nucleic acids that help store and use genetic information in cells. They are key molecules in biology and are often studied together because they work as a pair in living systems. Nucleic acids are built from repeating units called nucleotides. Each nucleotide has a sugar, a phosphate group, and a nitrogenous base. The order of these bases carries the genetic code. There are two main nucleic acids: DNA, or deoxyribonucleic acid, and RNA, or ribonucleic acid. DNA usually stores...

protein and protein structures

How Protein Shape Determines Function

Proteins are essential molecules in living things, and their shape affects how they work. Protein structure is described at several levels, including primary, secondary, tertiary, and quaternary structure. These levels help explain how a protein is built and how its final form supports its job. Primary structure is the sequence of amino acids in a protein. Secondary structure forms when parts of the chain fold into regular patterns. Tertiary structure is the overall three-dimensional shape of...

ph regulation in cells

Cell pH and Organelle Balance

Cell pH and organelle balance are essential for normal cell function. pH affects protein shape and activity, the charge on cell membranes, and many metabolic reactions inside the cell. Cells also use the proton motive force, which is the energy stored in a proton gradient, to make ATP, the cell’s main energy molecule. The cytosol, or fluid inside the cell, is usually slightly more acidic than the fluid outside the cell. Even so, cells must stop the cytosol from becoming too acidic. They use...

chemistry of the cell

Cell Chemistry: Water, Ions, and Molecules

Cell chemistry explains the substances that make cells work. Water is the main component of cells, and it supports the chemical reactions that keep life going. Cells also contain organic molecules, which are carbon-based compounds, and inorganic ions, which are charged particles needed for cell function. Organic molecules in cells include carbohydrates, lipids, proteins, and nucleic acids. These molecules have different roles, but together they build cell structures, store energy, and carry...

key terms and concepts

Explore DNA and RNA as nucleic acids, how nucleotides build them, and how these molecules store and use genetic information in cells.

DNA and RNA in Cells | JoVE K12

cell biology

JoVE Core Cell Biology illustrates complex cellular processes in easy-to-understand yet comprehensive animated video lessons. This collection focuses on the essential components and functions of cells, including organelles and membranes, cellular transport, and cell energetics. It is complemented by scientist-in-action videos that demonstrate the application of cell biology in real-world research experiments.

new-york-state-science-learning-standards

New York State Science Learning Standards

Structure and Function

HS-LS1-1.

Georgia Standards of Excellence

9-12

Biology

SB6.c

Characteristics of Life

Biology is a natural science that studies life and living organisms, including their structure, function, development, interactions, evolution, ...

https://cloudfront.jove.com/files/media/science-education/science-education-thumbs/10647.jpg

Cell Structure

Background
Cells represent the most basic biological units of all organisms, whether it be simple, single-celled organisms like bacteria, or large, ...

Visualizing Onion and Cheek Cells
ExpandImmediately before the experiment, wash and peel onion bulbs for the class.
Remove the entire brown outer skin and ...

Visualizing Onion and Human Cells
ExpandIn this experiment you will prepare different types of cells from a plant and animal, onion and human ...

What are Cells?

Cell Diversity

The concept of a cell started with microscopic observations of dead cork tissue by Robert Hooke in 1665. Hooke coined the term &#34;cell&#34; based on the ...

Cytoplasm

The cytoplasm consists of organelles and a framework of protein scaffolds called the cytoskeleton suspended in an aqueous solution, the cytosol. The ...

The Nucleus

The nucleus is a membrane-bound organelle that acts as a control center in a eukaryotic cell. It contains chromosomal DNA, which controls gene expression ...

The Central Dogma

The DNA Helix

DNA as a Genetic Template

Structure of a Gene

A gene is the fundamental unit of heredity. Every individual has two copies of each gene, one inherited from each parent. Although most people contain the ...

Chromosome Structure

Base-pairing and DNA Repair

What is Gene Expression?

A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised&#160; of nucleotides and proteins are ...

RNA Structure

The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form ...

Protein Families

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse ...

Conservation of Protein Domains

Transcription

Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. ...

Globular and Fibrous Proteins

Translation

Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes ...

Intrinsically Disordered Proteins

Ribosomes

Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that ...

Protein Complex Assembly

Protein and Protein Structures

Conjugated Proteins

Proteins: From Genes to Degradation

Amyloid Fibrils

What are Proteins?

Proteins are polymers of amino acids linked together by peptide bonds. Proteins and polypeptides are interchangeably used to refer to long chains of amino ...

Ligand Binding Sites

Protein Organization

Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular ...

Protein-Protein Interfaces

Protein Folding

Proteins are chains of amino acids linked together by peptide bonds. Upon synthesis, a protein folds into a three-dimensional conformation, critical to ...

Conserved Binding Sites

Cofactors and Coenzymes

Enzymes are proteins made of amino acids. The functional group of each constituent amino acid catalyzes a wide variety of chemical reactions via ionic ...

Cooperative Allosteric Transitions

Protein Kinases and Phosphatases

GTPases and their Regulation

Covalently Linked Protein Regulators

protein-complexes-with-interchangeable-parts

Protein Complexes with Interchangeable Parts

Mechanical Protein Function

Structural Protein Function

Protein Networks

Construct an explanation based on evidence for how the structure of DNA determines the structure of proteins, which carry out the essential functions of life through systems of specialized cells. [Assessment Boundary: Assessment does not include identification of specific cell or tissue types, whole body systems, specific protein structures and functions, or the biochemistry of protein synthesis.]

Levels of Organization

Biological organization is the classification of biological structures, ranging from atoms at the bottom of the hierarchy to the Earth&#x27;s biosphere. ...

Animal and Plant Cell Structure

Animal and plant cells not only differ in their structure, function, and mode of nutrition but also in how they reproduce, specialize, and organize into ...

structural-organization-of-the-human-body-an-overview

Structural Organization of the Human Body: An Overview

It is convenient to consider the body&#39;s structures in terms of fundamental levels of organization that increase in complexity: subatomic particles, ...

Overview of Human Digestion

The human body contains a monogastric digestive system. In a monogastric digestive system, the stomach only contains one chamber in which it digests food. ...

Anatomy of the Intestines

Although digestion of proteins, carbohydrates, and lipids may begin in the stomach, it is completed in the intestine. The absorption of nutrients, water, ...

Accessory Organs

Accessory organs are those that participate in the digestion of food but do not come into direct contact with it like the mouth, stomach, or intestine do. ...

Lipid Digestion

Lipids are large molecules that are generally not water-soluble. Since most of the digestive enzymes in the human body are water-based, there are specific ...

Protein Digestion

Protein digestion begins in the stomach, where the highly acidic environment can easily disrupt protein structure by exposing the peptide bonds of ...

Carbohydrate Digestion

Carbohydrate digestion and metabolism break down simple and complex carbohydrates from food into saccharides (i.e., sugars) for the body to use as energy. ...

What is a Nervous System?

Overview

The nervous system is the collection of specialized cells responsible for maintaining an organism&rsquo;s internal environment and coordinating ...

The Parasympathetic Nervous System

Overview

The parasympathetic nervous system is one of the two major divisions of the autonomic nervous system. This parasympathetic system is responsible ...

The Sympathetic Nervous System

Overview

The sympathetic nervous system&mdash;one of the two major divisions of the autonomic nervous system&mdash;is activated in times of stress. It ...

Oogenesis

In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from ...

What is a Sensory System?

Sensory systems detect stimuli&mdash;such as light and sound waves&mdash;and transduce them into neural signals that can be interpreted by the nervous ...

Fertilization

During fertilization, an egg and sperm cell fuse to create a new diploid structure. In humans, the process occurs once the egg has been released from the ...

What is the Skeletal System?

Overview

The adult human skeleton comprises 206 bones that are connected through cartilage, tendons, and ligaments. The skeleton provides a rigid ...

an-introduction-to-the-zebrafish-danio-rerio

An Introduction to the Zebrafish: Danio rerio

Zebrafish (Danio rerio) are small freshwater fish that are used as model organisms for biomedical research. The many strengths of these fish include their ...

Physiology of the Circulatory System

Homeostasis
Conditions in the external environment of an organism can change rapidly and drastically. To survive, organisms must maintain a fairly ...

Anatomy of the Circulatory System

The human circulatory system consists of blood, blood vessels that carry blood away from the heart, around the body, and back to the heart, and the heart ...

Measuring Blood Pressure
ExpandTo prepare for the blood pressure exercise, simply place the appropriate number of alcohol swabs, sphygmomanometers, and ...

Anatomy of the Heart

The human heart is made up of three layers of tissue that are surrounded by the pericardium, a membrane that protects and confines the heart. The ...

Measuring Circulatory System Function in Humans
ExpandBefore starting the experiment go to the front of the room and collect an alcohol swab, a ...

Blood Flow

Blood is pumped by the heart into the aorta, the largest artery in the body, and then into increasingly smaller arteries, arterioles, and capillaries. The ...

What Are Osmoregulation and Excretion?

Organisms must keep bodily fluids at a constant temperature and pH while maintaining specific solute concentrations in order to support life functions. ...

Blood Withdrawal I

Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN
Blood collection is a common requirement for ...

Kidney Structure

The kidneys are two large bean-shaped organs located in the upper abdomen. They filter the blood several times a day to remove toxins and rebalance water ...

Blood Withdrawal II

Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN
The collection of blood from mice and rats for ...

What is the Immune System?

Overview

The immune system comprises diverse biological structures and processes that protect the body from disease. These processes can be classified ...

Bone Remodeling

Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain ...

Spermatogenesis

Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of ...

Bone Structure

Within the skeletal system, the structure of a bone, or osseous tissue, can be exemplified in a long bone, like the femur, where there are two types of ...

Breathing

The process of breathing, inhaling and exhaling, involves the coordinated movement of the chest wall, the lungs, and the muscles that move them. Two ...

Develop and use a model to illustrate the hierarchical organization of interacting systems that provide specific functions within multicellular organisms. [Clarification Statement: Emphasis is on functions at the organism system level such as nutrient uptake, water delivery, and organism movement in response to neural stimuli. An example of an interacting system could be an artery depending on the proper function of elastic tissue and smooth muscle to regulate and deliver the proper amount of blood within the circulatory system.] [Assessment Boundary: Assessment does not include interactions and functions at the molecular or chemical reaction level.]

HS-LS1-2

DNA and RNA in Cells

Description

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