Laboratory studies investigating subsurface microbial processes, such as metal leaching in deep ore deposits (biomining), share common and challenging obstacles, including the special environmental conditions that need to be replicated, e.g., high pressure and in some cases acidic solutions. The former requires an experiment...

1. Preparation of the medium and inoculation of the microbial culture

1. Prepare a basal salt medium for autotrophic prokaryotes according to published techniques¹³. Dissolve and mix the chemicals below in distilled water (mg/L): Na₂SO₄·10H₂O (150) (NH₄