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In JoVE (1)
Other Publications (1)
Articles by Carol E. Soteropulos in JoVE
JoVE Bioengineering
Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents
Department of Biological Engineering, University of Missouri
Biosensors interface with complex, biological environments and perform targeted detection by combining highly sensitive sensors with highly specific probes attached to the sensor via surface modification. Here, we demonstrate the surface functionalization of silica optical sensors with biotin using silane coupling agents to bridge the sensor and the biological environment.
Other articles by Carol E. Soteropulos on PubMed
Determination of Binding Kinetics Using Whispering Gallery Mode Microcavities
Silica optical microcavity sensors show great promise in the kinetic evaluation of binding pairs, fundamental in understanding biomolecular interactions. Here, we develop and demonstrate a novel platform, based on bioconjugated silica microsphere resonators, to study the binding kinetics of the biotin-streptavidin system. We characterize the optical performance, verify the covalent attachment of biotin to the surface, and perform streptavidin detection experiments. We perform preliminary kinetic analysis of the detection data which shows the potential of whispering gallery mode resonators in the determination of the dissociation constant of the binding pair, which is in good agreement with previously published values.
