multimodal-3d-printing-of-phantoms-to-simulate-biological-tissue

Multimodal 3D Printing of Phantoms to Simulate Biological Tissue

Biomedical optical imaging is playing an important role in diagnosis and treatment of various diseases. However, the accuracy and the reproducibility of ...

University of Science and Technology of China

We introduce a microfluidic approach to simulate tumor hypoxia and vascular anomaly. Polydimethylsiloxane (PDMS) phantoms with embedded microchannel networks were fabricated by a soft lithography process. A dialysis membrane was sandwiched between two PDMS slabs to simulate the controlled mass transport and oxygen metabolism. A tortuous microchannel network was fabricated to simulate tumor microvasculature. A dual-modal multispectral and laser speckle imaging system was used for oxygen and blood flow imaging in the tumor-simulating phantom. The imaging results were compared with those of the normal vasculature. Our experiments demonstrated the technical feasibility of simulating tumor hypoxia and vascular anomalies using the proposed PDMS phantom. Such a phantom fabrication technique may be potentially used to calibrate optical imaging devices, to study the mechanisms for tumor hypoxia and angiogenesis, and to optimize the drug delivery strategies.

Microfabrication of polydimethylsiloxane phantoms to simulate tumor hypoxia and vascular anomaly.

Biomedical optical devices are widely used for clinical detection of various tissue anomalies. However, optical measurements have limited accuracy and traceability, partially owing to the lack of effective calibration methods that simulate the actual tissue conditions. To facilitate standardized calibration and performance evaluation of medical optical devices, we develop a three-dimensional fuse deposition modeling (FDM) technique for freeform fabrication of tissue-simulating phantoms. The FDM system uses transparent gel wax as the base material, titanium dioxide (TiO2 ) powder as the scattering ingredient, and graphite powder as the absorption ingredient. The ingredients are preheated, mixed, and deposited at the designated ratios layer-by-layer to simulate tissue structural and optical heterogeneities. By printing the sections of human brain model based on magnetic resonance images, we demonstrate the capability for simulating tissue structural heterogeneities. By measuring optical properties of multilayered phantoms and comparing with numerical simulation, we demonstrate the feasibility for simulating tissue optical properties. By creating a rat head phantom with embedded vasculature, we demonstrate the potential for mimicking physiologic processes of a living system.

Three-dimensional fuse deposition modeling of tissue-simulating phantom for biomedical optical imaging.

This paper describes the design, fabrication and locomotion of a starfish robot whose locomotion principle is derived from a starfish. The starfish robot has a number of tentacles or arms extending from its central body in the form of a disk, like the topology of a real starfish. The arm, which is a soft and composite structure (which we call the smart modular structure (SMS)) generating a planar reciprocal motion with a high speed of response upon the actuation provided by the shape memory alloy (SMA) wires, is fabricated from soft and smart materials. Based on the variation in the resistance of the SMA wires during their heating, an adaptive regulation (AR) heating strategy is proposed to (i) avoid overheating of the SMA wires, (ii) provide bending range control and (iii) achieve a high speed of response favorable to successfully propelling the starfish robot. Using a finite-segment method, a thermal dynamic model of the SMS is established to describe its thermal behavior under the AR and a constant heating strategy. A starfish robot with five SMS tentacles was tested with different control parameters to optimize its locomotion speed. As demonstrated in the accompanying video file, the robot successfully propelled in semi-submerged and underwater environments show its locomotion ability in the multi-media, like a real starfish. The propulsion speed of the starfish robot is at least an order of magnitude higher than that of those reported in the literature-thanks to the SMS controlled with the AR strategy.

A starfish robot based on soft and smart modular structure (SMS) actuated by SMA wires.

Preoperative assessment of tissue anatomy and accurate surgical planning is crucial in conjoined twin separation surgery. We developed a new method that combines three-dimensional (3D) printing, assembling, and casting to produce anatomic models of high fidelity for surgical planning. The related anatomic features of the conjoined twins were captured by computed tomography (CT), classified as five organ groups, and reconstructed as five computer models. Among these organ groups, the skeleton was produced by fused deposition modeling (FDM) using acrylonitrile-butadiene-styrene. For the other four organ groups, shell molds were prepared by FDM and cast with silica gel to simulate soft tissues, with contrast enhancement pigments added to simulate different CT and visual contrasts. The produced models were assembled, positioned firmly within a 3D printed shell mold simulating the skin boundary, and cast with transparent silica gel. The produced phantom was subject to further CT scan in comparison with that of the patient data for fidelity evaluation. Further data analysis showed that the produced model reassembled the geometric features of the original CT data with an overall mean deviation of less than 2 mm, indicating the clinical potential to use this method for surgical planning in conjoined twin separation surgery.

Freeform fabrication of tissue-simulating phantom for potential use of surgical planning in conjoined twins separation surgery.

Phantoms simulating polarization characteristics of soft tissue play an important role in the development, calibration, and validation of diagnostic polarized imaging devices and of therapeutic strategy, in both laboratory and clinical settings. We propose to fabricate optical phantoms that simulate polarization characteristics of dense fibrous tissues by bonding electrospun polylactic acid (PLA) fibers between polydimethylsiloxane (PDMS) substrate with a groove. Increasing the rotational speed of an electrospinning collector helps improve the orientation of the electrospun fibers. The phantoms simulate the polarization characteristics of dense fibrous tissue of collagenous fibroma and healthy skin with high fidelity. Our experiments demonstrate the technical potential of using such phantoms for validation and calibration of polarimetric medical devices.

Simulating orientation and polarization characteristics of dense fibrous tissue by electrostatic spinning of polymeric fibers.

Since tactile perception and robotic manipulation play important roles in human survival, we propose a new method for developing robotic tactile sensors based on the structural colours of Morpho menelaus (a kind of Morpho butterfly). The first task is to fabricate a flexible bioinspired grating with a similar microstructure to the wings of Morpho menelaus using the transfer technique, onto the surfaces of polydimethylsiloxane (PDMS) films. The second task, depending on the angle of diffracted light, is to integrate the flexible diffraction grating with a polychromatic light source and a CCD camera, and then predict the position and magnitude of the contact force caused by a change in the diffraction pattern. The final task is to set up an experimental calibration platform and a marker point array with an interval of 1 mm for an image processing algorithm and a deep learning method to establish the relationship between the contact point position, and the magnitude of the force and diffraction pattern. The results showed that this tactile sensor has high sensitivity and resolution, with the position of the contact force of 1 mm. This practical application of the UR-5 manipulator verifies the feasibility of the prototype as a tactile sensor. This tactile sensing method may be widely used in robotics by miniaturising the design.

Article	Year
Microfabrication of polydimethylsiloxane phantoms to simulate tumor hypoxia and vascular anomaly. Journal of biomedical optics\| PubMed ID: 26456687	2015
Three-dimensional fuse deposition modeling of tissue-simulating phantom for biomedical optical imaging. Journal of biomedical optics\| PubMed ID: 26603611	2015
A starfish robot based on soft and smart modular structure (SMS) actuated by SMA wires. Bioinspiration & biomimetics\| PubMed ID: 27609700	2016
Freeform fabrication of tissue-simulating phantom for potential use of surgical planning in conjoined twins separation surgery. Scientific reports\| PubMed ID: 28887492	2017
Simulating orientation and polarization characteristics of dense fibrous tissue by electrostatic spinning of polymeric fibers. Biomedical optics express\| PubMed ID: 30800500	2019
A new robotic tactile sensor with bio-mimetic structural colour inspired by Morpho butterflies. Bioinspiration & biomimetics\| PubMed ID: 31284276	2019

Erbao Dong

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