Jacek Otlewski Department of Protein Engineering, Faculty of Biotechnology University of Wroclaw Biography Publications Institution JoVE Articles Jacek Otlewski has not added a biography. If you are Jacek Otlewski and would like to personalize this page please email our Author Liaison for assistance. Publications FGF2-Derived PeptibodyF2-MMAE Conjugate for Targeted Delivery of Cytotoxic Drugs into Cancer Cells Overexpressing FGFR1 Cancers. Oct, 2020 | Pubmed ID: 33076489 Stable Fibroblast Growth Factor 2 Dimers with High Pro-Survival and Mitogenic Potential International Journal of Molecular Sciences. Jun, 2020 | Pubmed ID: 32526859 FGFR1 Clustering with Engineered Tetravalent Antibody Improves the Efficiency and Modifies the Mechanism of Receptor Internalization Molecular Oncology. 09, 2020 | Pubmed ID: 32511887 Site-Specific, Stoichiometric-Controlled, PEGylated Conjugates of Fibroblast Growth Factor 2 (FGF2) with Hydrophilic Auristatin Y for Highly Selective Killing of Cancer Cells Overproducing Fibroblast Growth Factor Receptor 1 (FGFR1) Molecular Pharmaceutics. 07, 2020 | Pubmed ID: 32501706 Intracellular Partners of Fibroblast Growth Factors 1 and 2 - Implications for Functions Cytokine & Growth Factor Reviews. May, 2020 | Pubmed ID: 32475760 FHF1 is a Bona Fide Fibroblast Growth Factor That Activates Cellular Signaling in FGFR-dependent Manner Cell Communication and Signaling : CCS. May, 2020 | Pubmed ID: 32357892 Low Stability of Integrin-Binding Deficient Mutant of FGF1 Restricts Its Biological Activity Cells. 08, 2019 | Pubmed ID: 31443196 Differential Regulation of Fibroblast Growth Factor Receptor 1 Trafficking and Function by Extracellular Galectins Cell Communication and Signaling : CCS. 06, 2019 | Pubmed ID: 31208421 Cross-Talk Between Fibroblast Growth Factor Receptors and Other Cell Surface Proteins Cells. 05, 2019 | Pubmed ID: 31091809 Crosstalk Between P38 and Erk 1/2 in Downregulation of FGF1-Induced Signaling International Journal of Molecular Sciences. Apr, 2019 | Pubmed ID: 31013829 Identification of a Peptide Antagonist of the FGF1-FGFR1 Signaling Axis by Phage Display Selection FEBS Open Bio. 05, 2019 | Pubmed ID: 30968602 Targeting Cellular Trafficking of Fibroblast Growth Factor Receptors As a Strategy for Selective Cancer Treatment Journal of Clinical Medicine. Dec, 2018 | Pubmed ID: 30577533 Correction: Serwotka-Suszczak, A. M. Et Al. A Conjugate Based on Anti-HER2 Diaffibody and Auristatin E Targets HER2-Positive Cancer Cells. Int. J. Mol. Sci. 2017, 18, 401 International Journal of Molecular Sciences. 11, 2018 | Pubmed ID: 30463379 Specific Antibody Fragment Ligand Traps Blocking FGF1 Activity International Journal of Molecular Sciences. Aug, 2018 | Pubmed ID: 30134556 Translocation of Exogenous FGF1 and FGF2 Protects the Cell Against Apoptosis Independently of Receptor Activation Journal of Molecular Biology. 10, 2018 | Pubmed ID: 30099027 FGF2 Dual Warhead Conjugate with Monomethyl Auristatin E and α-Amanitin Displays a Cytotoxic Effect Towards Cancer Cells Overproducing FGF Receptor 1 International Journal of Molecular Sciences. Jul, 2018 | Pubmed ID: 30029518 High Affinity Promotes Internalization of Engineered Antibodies Targeting FGFR1 International Journal of Molecular Sciences. May, 2018 | Pubmed ID: 29748524 Generation of High-affinity, Internalizing Anti-FGFR2 Single-chain Variable Antibody Fragment Fused with Fc for Targeting Gastrointestinal Cancers PloS One. 2018 | Pubmed ID: 29420662 Cytotoxic Conjugates of Fibroblast Growth Factor 2 (FGF2) with Monomethyl Auristatin E for Effective Killing of Cells Expressing FGF Receptors ACS Omega. Jul, 2017 | Pubmed ID: 30023704 Design and In Vitro Evaluation of a Cytotoxic Conjugate Based on the Anti-HER2 Affibody Fused to the Fc Fragment of IgG1 International Journal of Molecular Sciences. Aug, 2017 | Pubmed ID: 28771178 Antibody-induced Dimerization of FGFR1 Promotes Receptor Endocytosis Independently of Its Kinase Activity Scientific Reports. 08, 2017 | Pubmed ID: 28769084 The Autoinhibitory Function of D1 Domain of FGFR1 Goes Beyond the Inhibition of Ligand Binding The International Journal of Biochemistry & Cell Biology. 08, 2017 | Pubmed ID: 28652212 High-Yield Site-Specific Conjugation of Fibroblast Growth Factor 1 with Monomethylauristatin E Via Cysteine Flanked by Basic Residues Bioconjugate Chemistry. 07, 2017 | Pubmed ID: 28598150 Site-specific Conjugation of Fibroblast Growth Factor 2 (FGF2) Based on Incorporation of Alkyne-reactive Unnatural Amino Acid Bioorganic & Medicinal Chemistry. 07, 2017 | Pubmed ID: 28522266 High-Affinity Internalizing Human ScFv-Fc Antibody for Targeting FGFR1-Overexpressing Lung Cancer Molecular Cancer Research : MCR. 08, 2017 | Pubmed ID: 28483948 Anti-FGFR1 Aptamer-tagged Superparamagnetic Conjugates for Anticancer Hyperthermia Therapy International Journal of Nanomedicine. 2017 | Pubmed ID: 28442904 A Conjugate Based on Anti-HER2 Diaffibody and Auristatin E Targets HER2-Positive Cancer Cells International Journal of Molecular Sciences. Feb, 2017 | Pubmed ID: 28216573 Design and Characteristics of Cytotoxic Fibroblast Growth Factor 1 Conjugate for Fibroblast Growth Factor Receptor-targeted Cancer Therapy Drug Design, Development and Therapy. 2016 | Pubmed ID: 27563235 A Novel Affibody-Auristatin E Conjugate With a Potent and Selective Activity Against HER2+ Cell Lines Journal of Immunotherapy (Hagerstown, Md. : 1997). Jul-Aug, 2016 | Pubmed ID: 27227324 Identification of New FGF1 Binding Partners-Implications for Its Intracellular Function IUBMB Life. Mar, 2016 | Pubmed ID: 26840910 Selection of Specific Interactors from Phage Display Library Based on Sea Lamprey Variable Lymphocyte Receptor Sequences Biochimica Et Biophysica Acta. Dec, 2015 | Pubmed ID: 26391289 Selection and Characterization of Single Chain Antibody Fragments Specific for Hsp90 As a Potential Cancer Targeting Molecule International Journal of Molecular Sciences. Aug, 2015 | Pubmed ID: 26307975 Current Methods for the Synthesis of Homogeneous Antibody-drug Conjugates Biotechnology Advances. Nov, 2015 | Pubmed ID: 25981886 Efficient Production and Purification of Extracellular Domain of Human FGFR-Fc Fusion Proteins from Chinese Hamster Ovary Cells Protein Expression and Purification. Jul, 2014 | Pubmed ID: 24727156 Nucleolin Regulates Phosphorylation and Nuclear Export of Fibroblast Growth Factor 1 (FGF1) PloS One. 2014 | Pubmed ID: 24595027 Tumor-specific Hyperthermia with Aptamer-tagged Superparamagnetic Nanoparticles International Journal of Nanomedicine. 2014 | Pubmed ID: 24379664 Protease Resistant Variants of FGF1 with Prolonged Biological Activity Protein and Peptide Letters. May, 2014 | Pubmed ID: 24304385 Design, Expression and Characterization of a Highly Stable Tetratricopeptide-based Protein Scaffold for Phage Display Application Acta Biochimica Polonica. 2013 | Pubmed ID: 24350305 Aptamers: Molecules of Great Potential Biotechnology Advances. Dec, 2013 | Pubmed ID: 23632375 FGF1-gold Nanoparticle Conjugates Targeting FGFR Efficiently Decrease Cell Viability Upon NIR Irradiation International Journal of Nanomedicine. 2012 | Pubmed ID: 23226697 A Novel, Stable, Helical Scaffold As an Alternative Binder - Construction of Phage Display Libraries Acta Biochimica Polonica. 2012 | Pubmed ID: 23032749 Femtomolar Zn2+ Affinity of LIM Domain of PDLIM1 Protein Uncovers Crucial Contribution of Protein-protein Interactions to Protein Stability Journal of Inorganic Biochemistry. Oct, 2012 | Pubmed ID: 22922308 Selection and Characterization of Human Antibody Fragments Specific for Psoriasin - a Cancer Associated Protein Biochemical and Biophysical Research Communications. Mar, 2012 | Pubmed ID: 22342672 Tailoring Small Proteins Towards Biomedical Applications Current Pharmaceutical Biotechnology. Nov, 2011 | Pubmed ID: 21902634 PDZ Domain from Dishevelled -- a Specificity Study Acta Biochimica Polonica. 2011 | Pubmed ID: 21666888 Design and Characteristics of a Stable Protein Scaffold for Specific Binding Based on Variable Lymphocyte Receptor Sequences Biochimica Et Biophysica Acta. Sep, 2011 | Pubmed ID: 21621013 Longer Action Means Better Drug: Tuning Up Protein Therapeutics Biotechnology Advances. Jul-Aug, 2011 | Pubmed ID: 21443940 The High Zn(II) Affinity of the Tetracysteine Tag Affects Its Fluorescent Labeling with Biarsenicals Chembiochem : a European Journal of Chemical Biology. Jun, 2010 | Pubmed ID: 20440728 Increased Protein Stability of FGF1 Can Compensate for Its Reduced Affinity for Heparin The Journal of Biological Chemistry. Sep, 2009 | Pubmed ID: 19574212 Structure of a Highly Stable Mutant of Human Fibroblast Growth Factor 1 Acta Crystallographica. Section D, Biological Crystallography. Jan, 2009 | Pubmed ID: 19153468 Dissecting the Thermodynamics of GAP-RhoA Interactions Journal of Structural Biology. Jan, 2009 | Pubmed ID: 18929667 Degenerate Specificity of PDZ Domains from RhoA-specific Nucleotide Exchange Factors PDZRhoGEF and LARG Acta Biochimica Polonica. 2008 | Pubmed ID: 18542831 Expression, Purification and Crystallization of Cysteine-rich Human Protein Muskelin in Escherichia Coli Protein Expression and Purification. Jul, 2008 | Pubmed ID: 18455433 Bivalent Peptides As Models for Multimeric Targets of PDZ Domains Chembiochem : a European Journal of Chemical Biology. Mar, 2007 | Pubmed ID: 17279591 Structural Requirements of FGF-1 for Receptor Binding and Translocation into Cells Biochemistry. Dec, 2006 | Pubmed ID: 17176056 Novel Peptide Recognized by RhoA GTPase Acta Biochimica Polonica. 2006 | Pubmed ID: 17019437 Crystal Structure of Vigna Radiata Cytokinin-specific Binding Protein in Complex with Zeatin The Plant Cell. Oct, 2006 | Pubmed ID: 16998071 The Molecular Basis of RhoA Specificity in the Guanine Nucleotide Exchange Factor PDZ-RhoGEF The Journal of Biological Chemistry. Oct, 2006 | Pubmed ID: 16954208 The Dimerization Mechanism of LIS1 and Its Implication for Proteins Containing the LisH Motif Journal of Molecular Biology. Mar, 2006 | Pubmed ID: 16445939 Highly Stable Mutants of Human Fibroblast Growth Factor-1 Exhibit Prolonged Biological Action Journal of Molecular Biology. Sep, 2005 | Pubmed ID: 16126225 The Many Faces of Protease-protein Inhibitor Interaction The EMBO Journal. Apr, 2005 | Pubmed ID: 15775973 Crystal Structures of Five Bovine Chymotrypsin Complexes with P1 BPTI Variants Journal of Molecular Biology. Dec, 2004 | Pubmed ID: 15544809 Design of Fully Active FGF-1 Variants with Increased Stability Protein Engineering, Design & Selection : PEDS. Aug, 2004 | Pubmed ID: 15469994 Trypsin Specificity As Elucidated by LIE Calculations, X-ray Structures, and Association Constant Measurements Protein Science : a Publication of the Protein Society. Apr, 2004 | Pubmed ID: 15044735 PDZ Domains - Common Players in the Cell Signaling Acta Biochimica Polonica. 2003 | Pubmed ID: 14739991 Amyloid-forming Peptides Selected Proteolytically from Phage Display Library Protein Science : a Publication of the Protein Society. Aug, 2003 | Pubmed ID: 12876317 Importance of Alpha-helix N-capping Motif in Stabilization of Betabetaalpha Fold Protein Science : a Publication of the Protein Society. Jun, 2003 | Pubmed ID: 12761399 Hydrogen Bonds in Human Ubiquitin Reflected in Temperature Coefficients of Amide Protons Journal of Magnetic Resonance (San Diego, Calif. : 1997). Aug, 2002 | Pubmed ID: 12323135 NMR Structures of Two Variants of Bovine Pancreatic Trypsin Inhibitor (BPTI) Reveal Unexpected Influence of Mutations on Protein Structure and Stability Journal of Molecular Biology. Aug, 2002 | Pubmed ID: 12206780 Thermodynamics of Single Peptide Bond Cleavage in Bovine Pancreatic Trypsin Inhibitor (BPTI) Protein Science : a Publication of the Protein Society. Apr, 2002 | Pubmed ID: 11910035 Analysis of Serine Proteinase-inhibitor Interaction by Alanine Shaving Protein Science : a Publication of the Protein Society. Apr, 2002 | Pubmed ID: 11910024 Amino-acid Substitutions at the Fully Exposed P1 Site of Bovine Pancreatic Trypsin Inhibitor Affect Its Stability Protein Science : a Publication of the Protein Society. Apr, 2001 | Pubmed ID: 11274462 Predicting the Reactivity of Proteins from Their Sequence Alone: Kazal Family of Protein Inhibitors of Serine Proteinases Proceedings of the National Academy of Sciences of the United States of America. Feb, 2001 | Pubmed ID: 11171964 Preparation of Site-Specific Cytotoxic Protein Conjugates via Maleimide-thiol Chemistry and Sortase A-Mediated Ligation Mateusz Adam Krzyscik1,2, Aleksandra Sokolowska-Wedzina1, Karolina Jendryczko1, Marta Pozniak1, Daria Nawrocka1, Natalia Porebska1, Malgorzata Zakrzewska1, Jacek Otlewski1, Anna Szlachcic1, Lukasz Opalinski1 1Department of Protein Engineering, Faculty of Biotechnology, University of Wroclaw, 2Department of Protein Biotechnology, Faculty of Biotechnology, University of Wroclaw JoVE 61918 Biochemistry
Preparation of Site-Specific Cytotoxic Protein Conjugates via Maleimide-thiol Chemistry and Sortase A-Mediated Ligation Mateusz Adam Krzyscik1,2, Aleksandra Sokolowska-Wedzina1, Karolina Jendryczko1, Marta Pozniak1, Daria Nawrocka1, Natalia Porebska1, Malgorzata Zakrzewska1, Jacek Otlewski1, Anna Szlachcic1, Lukasz Opalinski1 1Department of Protein Engineering, Faculty of Biotechnology, University of Wroclaw, 2Department of Protein Biotechnology, Faculty of Biotechnology, University of Wroclaw JoVE 61918 Biochemistry