Articles by Jonas Waldenström in JoVE

Avian Influenza Surveillance with FTA Cards: Field Methods, Biosafety, and Transportation Issues Solved

JoVE 2832 8/02/2011

Robert H.S. Kraus¹, Pim van Hooft¹, Jonas Waldenström², Neus Latorre-Margalef², Ronald C. Ydenberg^1,3, Herbert H.T. Prins¹

¹Resource Ecology Group, Wageningen University, ²Section for Zoonotic Ecology and Epidemiology, School of Natural Sciences, Linnaeus University, ³Centre for Wildlife Ecology, Simon Fraser University

A method to preserve, detect and sequence RNA from Avian Influenza Viruses was validated and extended using natural faecal samples from birds. This technique removes the necessity of maintaining a cool chain and handling of infectious viruses and can be applied in a 96-well high-throughput setup.