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In JoVE (1)
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Articles by Laura Molloy in JoVE
JoVE General
A metilação do DNA: Modificação e Análise de bissulfito
1Epigenetics Group, Cancer Research Program, Garvan Institute of Medical Research, 2St Vincent's Clinical School, University of NSW
O padrão ouro para a análise de metilação do DNA é sequenciamento genômico de DNA convertidos bissulfito. Este método tira vantagem do aumento da sensibilidade em comparação com 5 citosina-metilcitosina (5-MEC) para bissulfito de desaminação em condições ácidas. Citosinas não metiladas pode ser distinguida de citosinas metiladas após a amplificação do DNA genômico alvo.
Other articles by Laura Molloy on PubMed
Surgically Induced Astigmatism After Phacoemulsification with and Without Correction for Posture-related Ocular Cyclotorsion: Randomized Controlled Study
To report the impact of posture-related ocular cyclotorsion on one surgeon's surgically induced astigmatism (SIA) results and the variance in SIA.
Periconceptional Undernutrition in Normal and Overweight Ewes Leads to Increased Adrenal Growth and Epigenetic Changes in Adrenal IGF2/H19 Gene in Offspring
Adverse conditions in early life result in increased activation of the hypothalamo-pituitary-adrenal axis and in stress responsiveness in offspring. We have developed a model in which "donor" ewes are either normally nourished or overnourished prior to a period of dietary restriction, before transfer of the embryo at 6-7 d after conception to a ewe of normal weight and nutritional history. A moderate restriction of energy intake during the periconceptional period in both normal weight and overweight ewes resulted in increased adrenal mass in male and female lambs and an increased cortisol response to stress in female lambs. The increase in adrenal weight in lambs exposed to periconceptional undernutrition was associated with a decrease in the adrenal mRNA expression of IGF2 and decreased methylation in the proximal CTCF-binding site in the differentially methylated region of the IGF2/H19 gene. Thus, weight loss in both normal and overweight mothers during the periconceptional period results in epigenetic modification of IGF2 in the adrenal gland, adrenal overgrowth, and increased vulnerability to stress in offspring. Determining the appropriate approach to weight loss in the periconceptional period may therefore be important in overweight or obese women seeking to become pregnant.
Value of Dual Biometry in the Detection and Investigation of Error in the Preoperative Prediction of Refractive Status Following Cataract Surgery
To report the value of dual biometry in the detection of biometry errors.
Estimation of Effective Lens Position Using a Method Independent of Preoperative Keratometry Readings
To evaluate the validity of a keratometry (K)-independent method of estimating effective lens position (ELP) before phacoemulsification cataract surgery.
