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In JoVE (1)
Other Publications (2)
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Articles by Stylianos P. Scordilis in JoVE
प्रोटिओमिक्स से कंकाल की मांसपेशी लैंगिक द्विरूपता
Kalina Dimova1, Lauren Ann Metskas2, Mohini Kulp3, Stylianos P. Scordilis4
1Center for Proteomics, Smith College, 2Department of Molecular Biophysics and Biochemistry, Yale University, 3Department of Chemistry, Smith College, 4Department of Biological Sciences and Center for Proteomics, Smith College
तरीकों का एक सेट सीधे आगे के लिए अलग और सबसे प्रचुर मात्रा में प्रोटीन के कंकाल की मांसपेशी में व्यक्त पहचान निर्धारित है. 800 स्पॉट के बारे में 10 मिलीग्राम मांसपेशियों से एक दो आयामी जेल पर discerned हैं, यह लिंग विशिष्ट प्रोटीन अभिव्यक्ति का निर्धारण करने के लिए अनुमति देता है. इन विधियों सबसे ऊतकों में बराबर परिणाम दे देंगे.
Other articles by Stylianos P. Scordilis on PubMed
Serum Creatine Kinase Activity Varies with Ovulatory Status in Regularly Exercising, Premenopausal Women
Hormone Research. 2006 | Pubmed ID: 16514242
The clinical complications associated with an unopposed estrogen environment and luteal phase defects observed in exercising women prompted the examination of the relationship of exercise and endogenous ovarian steroids with serum creatine kinase (CK) activity.
Cellular & Molecular Biology Letters. Sep, 2010 | Pubmed ID: 20563704
Skeletal muscle is a plastic tissue with known gender dimorphism, especially at the metabolic level. A proteomic comparison of male and female murine biceps brachii was undertaken, resolving an average of 600 protein spots of MW 15-150 kDa and pI 5-8. Twenty-six unique full-length proteins spanning 11 KOG groups demonstrated statistically significant (p<0.05) abundance differences between genders; the majority of these proteins have metabolic functions. Identified glycolytic enzymes demonstrated decreased abundance in females, while abundance differences in identified oxidative phosphorylation enzymes were specific to the proteins rather than to the functional group as a whole. Certain cytoskeletal and stress proteins showed specific expression differences, and all three phosphorylation states of creatine kinase showed significant decreased abundance in females. Expression differences were significant but many were subtle (< or = 2-fold), and known hormonally-regulated proteins were not identified. We conclude that while gender dimorphism is present in non-exercised murine skeletal muscle, the proteome comparison of male and female biceps brachii in exercise-naive mice indicates subtle differences rather than a large or obviously hormonal dimorphism.