a-simplified-technique-for-situ-excision-cornea-evisceration-retinal

A Simplified Technique for <em>In situ</em> Excision of Cornea and Evisceration of Retinal Tissue from Human Ocular Globe

Enucleation is the process of retrieving the ocular globe from a cadaveric donor leaving the rest of the globe undisturbed. Excision refers to the ...

Fondazione Banca Degli Occhi del Veneto O.N.L.U.S. 

Human limbal stem cells produce transit amplifying progenitors that migrate centripetally to regenerate the corneal epithelium. Coexpression of CCAAT enhancer binding protein delta (C/EBPdelta), Bmi1, and DeltaNp63alpha identifies mitotically quiescent limbal stem cells, which generate holoclones in culture. Upon corneal injury, a fraction of these cells switches off C/EBPdelta and Bmi1, proliferates, and differentiates into mature corneal cells. Forced expression of C/EBPdelta inhibits the growth of limbal colonies and increases the cell cycle length of primary limbal cells through the activity of p27(Kip1) and p57(Kip2). These effects are reversible; do not alter the limbal cell proliferative capacity; and are not due to apoptosis, senescence, or differentiation. C/EBPdelta, but not DeltaNp63alpha, indefinitely promotes holoclone self-renewal and prevents clonal evolution, suggesting that self-renewal and proliferation are distinct, albeit related, processes in limbal stem cells. C/EBPdelta is recruited to the chromatin of positively (p27(Kip1) and p57(Kip2)) and negatively (p16(INK4A) and involucrin) regulated gene loci, suggesting a direct role of this transcription factor in determining limbal stem cell identity.

Article	Year
Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 15983386	2005
Microscopy research and technique\| PubMed ID: 16972233	2006
Investigative ophthalmology & visual science\| PubMed ID: 17122109	2006
C/EBPdelta regulates cell cycle and self-renewal of human limbal stem cells. The Journal of cell biology\| PubMed ID: 17562792	2007
The British journal of ophthalmology\| PubMed ID: 19740872	2010
Molecular vision\| PubMed ID: 19862337	2009
The British journal of ophthalmology\| PubMed ID: 20494912	2010
Experimental eye research\| PubMed ID: 20537995	2010
The ocular surface\| PubMed ID: 20712971	2010
Clinical & experimental ophthalmology\| PubMed ID: 21668791	2012
Ophthalmology\| PubMed ID: 21959367	2012
The Journal of molecular diagnostics : JMD\| PubMed ID: 22056627	2012
Current genomics\| PubMed ID: 22131869	2011

Vanessa Barbaro

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