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Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment (Video) | JoVE
Multiplex Immunofluorescence Staining and Image Analysis (Video) | JoVE
本文描述了一种手动酪胺信号放大(TSA)多重免疫荧光(mIF)结合图像分析和空间分析的方案。该方案可与福尔马林固定石蜡包埋(FFPE)切片一起使用,根据实验室中可用的玻片扫描仪,每张载玻片可染色两到六种抗原。
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