Expanding the Scope of Multiplex Immunoassays for Lyme Borreliosis Diagnostics and Pathogen Research (Video) | JoVE

Julia Häring; Tanja Michel; Matthias Becker; Daniel Junker; Tatia Tchitchagua; Olaf Leschnik; Berit Lange; Stefanie Castell; Gérard Krause; Monika Strengert; Alex Dulovic; Nicole Schneiderhan-Marra

doi:10.3791/65323

Multiplex Immunoassay for Borrelia-Specific IgG and IgM in Serum

July 14th, 2023

Expanding the Scope of Multiplex Immunoassays for Lyme Borreliosis Diagnostics and Pathogen Research (Video) | JoVE

01:59 min

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Dual-Reporter IgG and IgM Serological Assay for Simultaneous Antibody Detection of Borrelia (Video) | JoVE

03:29 min

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Julia Häring*¹, Tanja Michel*¹, Matthias Becker¹, Daniel Junker¹, Tatia Tchitchagua², Olaf Leschnik², Berit Lange^3,4, Stefanie Castell^3,4, Gérard Krause^3,4, Monika Strengert³, Alex Dulovic¹, Nicole Schneiderhan-Marra¹

¹NMI Natural and Medical Sciences Institute at the University of Tübingen, ²Department of Neurology, Sächsisches Krankenhaus Rodewisch, ³Department of Epidemiology, Helmholtz Centre for Infection Research, ⁴German Centre for Infection Research (DZIF)

* These authors contributed equally

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A three-channel dual-reporter fluorescence flow analysis system was used to develop a bead-based multiplex immunoassay that simultaneously evaluates serum samples for IgG and IgM elicited against multiple antigens of different Borrelia species that cause Lyme borreliosis in Europe and North America.

Article

Multiplex Immunoassay for Borrelia-Specific IgG and IgM in Serum

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