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Importance of Single Cell Sorting in Isolating Purified Populations of Mesenchymal Stem Cells (Video) | JoVE
A Step-by-Step Guide for Seeding and Differentiation of Mesenchymal Stem Cell Cultures for Characterization (Video) | JoVE
Lineage-Specific Cytochemical Staining of Mesenchymal Stem Cells for Characterizing Differentiated Cells (Video) | JoVE
Mesenchymal Stem Cell Surface Staining for Immunophenotyping (Video) | JoVE
Mesenchymal Stem Cell Sample Preparation, Flow Cytometric Analysis, and Cell Sorting (Video) | JoVE
该协议描述了使用单细胞分选方法对人间充质干细胞进行荧光激活细胞分选的使用。具体而言,当与基于多参数流式细胞术的方法相结合时,使用单细胞分选可以实现来自异质群体的免疫表型细胞的 99% 纯度。
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