Fluorescence-Activated Single-Cell Sorting of Human Mesenchymal Stem Cells

November 10th, 2023

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Ayona Gupta*¹, Risani Mukhopadhyay*¹, Himanshi Khandelwal¹, Narendra Nala², Uttara Chakraborty¹

¹Manipal Institute of Regenerative Medicine, Bengaluru, Manipal Academy of Higher Education, Manipal, ²HIV-AIDS Laboratory, Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research (JNCASR)

* These authors contributed equally

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Denne protokol beskriver brugen af fluorescensaktiveret cellesortering af humane mesenkymale stamceller ved hjælp af enkeltcellesorteringsmetoden. Specifikt kan brugen af enkeltcellesortering opnå 99% renhed af de immunofænotypede celler fra en heterogen population, når den kombineres med en multiparametrisk flowcytometribaseret tilgang.

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Fluorescence-Activated Single-Cell Sorting of Human Mesenchymal Stem Cells

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