Fluorescence-Activated Single-Cell Sorting of Human Mesenchymal Stem Cells

November 10th, 2023

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Ayona Gupta*¹, Risani Mukhopadhyay*¹, Himanshi Khandelwal¹, Narendra Nala², Uttara Chakraborty¹

¹Manipal Institute of Regenerative Medicine, Bengaluru, Manipal Academy of Higher Education, Manipal, ²HIV-AIDS Laboratory, Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research (JNCASR)

* These authors contributed equally

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Questo protocollo descrive l'uso della selezione cellulare attivata dalla fluorescenza di cellule staminali mesenchimali umane utilizzando il metodo di selezione a singola cellula. In particolare, l'uso del sorting a singola cellula può raggiungere una purezza del 99% delle cellule immunofenotipizzate da una popolazione eterogenea se combinato con un approccio basato sulla citometria a flusso multiparametrica.

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Fluorescence-Activated Single-Cell Sorting of Human Mesenchymal Stem Cells

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