Unveiling the Pathway Linking Obesity to Autoimmune Inflammation in Multiple Sclerosis (Video) | JoVE

Carmen C. Ucciferri; Annette Gower; Nuria Alvarez-Sanchez; Heather Whetstone; Valeria Ramaglia; Jennifer L. Gommerman; Koroboshka Brand-Arzamendi; Raphael Schneider; Shannon E Dunn

doi:10.3791/65738

Histological Staining of Spinal Cord for Assessing EAE Disease Severity

February 23rd, 2024

Unveiling the Pathway Linking Obesity to Autoimmune Inflammation in Multiple Sclerosis (Video) | JoVE

01:51 min

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Harvesting and Fixing Mouse Brain and Spinal Cord Tissues for Histological Scoring of Experimental Autoimmune Encephalomyelitis (Video) | JoVE

01:30 min

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Paraffin Embedding and Histopathological Scoring of Demyelinating Lesions and Perivascular Cuffs in Mouse Brain and Spinal Cord Tissues (Video) | JoVE

05:04 min

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Carmen C. Ucciferri¹, Annette Gower², Nuria Alvarez-Sanchez^1,2, Heather Whetstone³, Valeria Ramaglia¹, Jennifer L. Gommerman¹, Koroboshka Brand-Arzamendi², Raphael Schneider^2,4, Shannon E Dunn^1,2,5

¹Department of Immunology, University of Toronto, ²Keenan Research Centre for Biomedical Science of St. Michael’s Hospital, ³Sickkids Research Institute, The Hospital for Sick Children, ⁴BARLO MS Centre, St. Michael’s Hospital, ⁵Women’s College Research Institute, Women’s College Hospital

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Experimental autoimmune encephalomyelitis (EAE) serves as an animal model of multiple sclerosis. This article describes an approach for scoring spinal cord inflammation, demyelination, and axonal injury in EAE. Additionally, a method to quantify soluble neurofilament light levels in the mice serum is presented, facilitating the assessment of axonal injury in live mice.

Article

Histological Staining of Spinal Cord for Assessing EAE Disease Severity

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