JoVE Chemistry surveys a broad scope of chemical disciplines. This collection includes fundamental and applied research that investigates molecular interactions, properties, and structure spanning in the following core divisions: analytical, organic, organometallic, inorganic, bioinorganic, and biochemistry. This section also includes the design and preparation of materials and small molecules for advanced or unique applications.
1School of Pharmacy, Pacific University, 2Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University
The goal of this protocol is to describe the preparation and characterization of physically entrapped, poorly water soluble drugs in micellar drug delivery systems composed of amphiphilic block copolymers.
Published August 28, 2015. Keywords: Chemistry, Amphiphilic block copolymers, Polymeric micelles, drug delivery, in vitro characterization, chemotherapy, multi-drug loading, nanocarriers
1Department of Materials Science and Engineering, Clemson University, 2Department of Physics and Astronomy, Clemson University, 3Electron Microscope Facility, Clemson University, 4Materials Science and Engineering, King Abdullah University of Science and Technology
A protocol for the synthesis and processing of polycrystalline SrTiO3 ceramics doped non-uniformly with Pr is presented along with the investigation of their thermoelectric properties.
Published August 15, 2015. Keywords: Chemistry, Oxide Thermoelectrics, Praseodymium, SrTiO3, Enhanced Figure of Merit, Enhanced Power Factor, N-type Semiconductor, Mobility Enhancement, Grain Boundary Engineering
1Environmental Health Science and Research Bureau, Health Canada
The goal of this protocol is the detection of the DNA oxidation marker, 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dGuo) by HPLC-ED, in DNA from cultured cells or animal tissues.
Published August 1, 2015. Keywords: Chemistry, Oxidative Stress, DNA Damage, 8-oxo-7, 8-dihydro-2'-deoxyguanosine, 8-hydroxy-2'-deoxyguanosine, Xenobiotic Metabolism, Human Health
1Department of Chemistry and Biochemistry, University of Notre Dame, 2Department of Chemical and Biomolecular Engineering, University of Notre Dame, 3Department of Chemistry, Physics, and Engineering Studies, Chicago State University, 4Department of Technology, Ivy Tech Community College, South Bend, Indiana
Reproducible cleaning processes for substrates used in DNA origami research are described, including bench-top RCA cleaning and derivatization of silicon oxide. Protocols for surface preparation, DNA origami deposition, drying parameters, and simple experimental set-ups are illustrated.
Published July 23, 2015. Keywords: Chemistry, Nanostructures, substrate, DNA origami, mica, silicon, biomolecule, self-assembly
1Battelle Center for Mathematical Medicine, The Research Institute at Nationwide Children's Hospital
Synthetic protein sequences based on consensus motifs typically ignore co-evolving residues, that imply interpositional dependencies (IPDs). IPDs can be essential to activity, and designs that disregard them may result in suboptimal results. This protocol uses StickWRLD to identify IPDs and help inform rational protein design, resulting in more efficient results.
Published July 14, 2015. Keywords: Chemistry, protein engineering, covariation, codependent residues, visualization
1The Molecular Foundry, E. O. Lawrence Berkeley National Laboratory, 2Institute of Chemical Sciences, Bahauddin Zakariya University, 3Department of Chemistry, University of the Balearic Islands, 4Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology
A procedure for the preparation of porous hybrid separation media composed of a macroporous polymer monolith internally coated by a high surface area microporous coordination polymer is presented.
Published July 14, 2015. Keywords: Chemistry, Porous materials, hybrid materials, polymer monoliths, porous coordination polymers, flow-through supports, phosphopeptide enrichment, mass spectrometry
1Division of Chemistry and Biological Chemistry, Nanyang Technological University
We report protocols for “polymerizing” various types of polymer-encapsulated metal nanoparticles into long chains of “homo-“ and “co-polymers”.
Published July 9, 2015. Keywords: Chemistry, Nanoparticle, chain, self-assembly, encapsulation, polymer, homo-polymerization, co-polymerization, polystyrene-block-poly(acrylic acid)
1Department of Chemistry and Geochemistry, Colorado School of Mines
Here, we present a protocol with a sol-gel process to synthesize gold intercalated in the walls of mesoporous materials (GMS), which is confirmed to possess a mesoporous matrix with gold intercalated in the walls imparting great stability and recyclability.
Published July 9, 2015. Keywords: Chemistry, Gold, intercalated, silica, mesoporous material, nano reactor, thermally stable, catalyst, recyclable
1Department of Chemistry, Vanderbilt University
Robust detection reagents are of increasing necessity for developing new malaria diagnostic tools. An iridium(III) probe was designed that emits long-lasting luminescent signal in the presence of a histidine-rich malarial protein biomarker. Detection of the protein either in solution or immobilized on a magnetic particle affords flexibility in application.
Published July 7, 2015. Keywords: Chemistry, Histidine-rich protein, luminescence, metal-based probe, magnetic separation, ELISA, protein labeling, malaria
1Department of Physics, The Ohio State University, 2Department of Chemistry, The Ohio State University
We present the synthesis of the organic-based ferrimagnet vanadium tetracyanoethylene (V[TCNE]x, x~2) via low temperature chemical vapor deposition (CVD). This optimized recipe yields an increase in Curie temperature from 400 K to over 600 K and a dramatic improvement in magnetic resonance properties.
Published July 3, 2015. Keywords: Chemistry, organic-based magnet, thin film, room temperature, spintronics, magnetism, chemical vapor deposition