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General Laboratory Techniques

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Basic Methods in Cellular and Molecular Biology

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JoVE Chemistry
JoVE Chemistry surveys a broad scope of chemical disciplines. This collection includes fundamental and applied research that investigates molecular interactions, properties, and structure spanning in the following core divisions: analytical, organic, organometallic, inorganic, bioinorganic, and biochemistry. This section also includes the design and preparation of materials and small molecules for advanced or unique applications.
 JoVE Chemistry

Synthesis of Indoxyl-glycosides for Detection of Glycosidase Activities

1Department of Chemistry, Faculty of Sciences, University of Hamburg


JoVE 52442

Indoxyl glycosides are well-established and widely used tools for enzyme screening and enzyme activity monitoring. Especially for glucose type structures previous syntheses proved to be challenging and low yielding. Our novel approach employs indoxylic acid esters as precious intermediates to yield a considerable number of indoxyl glycosides in good yields.

 JoVE Chemistry

High Precision Zinc Isotopic Measurements Applied to Mouse Organs

1Institut de Physique du Globe de Paris, Institut Universitaire de France, Université Paris Diderot, 2INSERM (Institut National de la Santé et de la Recherche Médicale), U1148: Laboratory for Vascular Translational Science, Université Paris Diderot


JoVE 52479

We present the technique to measure with high precision zinc isotope ratios in mouse organs.

 JoVE Chemistry

Supercritical Nitrogen Processing for the Purification of Reactive Porous Materials

1Hydrogen and Energy Laboratory, Empa, Swiss Federal Laboratories for Materials Science and Technology


JoVE 52817

Nitrogen is an effective supercritical fluid for extraction or drying processes due to its small molecular size, high density in the near-liquid supercritical regime, and chemical inertness. We present a supercritical nitrogen drying protocol for the purification treatment of reactive, porous materials.

 JoVE Chemistry

Diffuse Reflectance Infrared Spectroscopic Identification of Dispersant/Particle Bonding Mechanisms in Functional Inks

1Department of Chemistry, New York City College of Technology, City University of New York (CUNY)


JoVE 52744

Formulation of stable, functional inks is critical to expanding the applications of additive manufacturing. In turn, knowledge of the mechanisms of dispersant/particle bonding is required for effective ink formulation. Diffuse Reflectance Fourier Transform Infrared Spectroscopy (DRIFTS) is presented as a simple, inexpensive way to gain insight into these mechanisms.

 JoVE Chemistry

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

1Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, 2Wyss Institute for Biologically Inspired Engineering, Harvard University, 3Department of Systems Biology, Harvard Medical School


JoVE 52486

DNA tiling is an effective approach to make programmable nanostructures. We describe the protocols to construct complex two-dimensional shapes by the self-assembly of single-stranded DNA tiles.

 JoVE Chemistry

Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders

1Department of Industrial and Physical Pharmacy, Purdue University


JoVE 52503

Here, we present detailed protocols for solid-state amide hydrogen/deuterium exchange mass spectrometry (ssHDX-MS) and solid-state photolytic labeling mass spectrometry (ssPL-MS) for proteins in solid powders. The methods provide high-resolution information on protein conformation and interactions in the amorphous solid-state, which may be useful in formulation design.

 JoVE Chemistry

The Synthesis, Characterization and Reactivity of a Series of Ruthenium N-triphosPh Complexes

1Department of Chemistry, Imperial College London


JoVE 52689

Ruthenium phosphine complexes are widely used for homogeneous catalytic reactions such as hydrogenations. The synthesis of a series of novel tridentate ruthenium complexes bearing the N-triphos ligand N(CH2PPh2)3 is reported. Additionally, the stoichiometric reaction of a dihydride Ru–N-triphos complex with levulinic acid is described.

 JoVE Chemistry

Sedimentation Equilibrium of a Small Oligomer-forming Membrane Protein: Effect of Histidine Protonation on Pentameric Stability

1School of Biological Sciences, Nanyang Technological University


JoVE 52404

Sedimentation equilibrium (SE) can be used to study protein-protein interactions in a physiological environment. This manuscript describes the use of this technique to determine the effect of pH on the stability of a homo-pentamer formed by the small hydrophobic (SH) protein encoded by the human syncytial respiratory virus (hRSV).

 JoVE Chemistry

Transport of Surface-modified Carbon Nanotubes through a Soil Column

1School of Ecology and Environmental Studies, Nalanda University, 2Department of Earth Sciences, Uppsala University


JoVE 52634

Surface properties of a nanoparticle are important for their interaction with the surrounding medium. Therefore the surface modification of carbon nanotubes can be critical for their transport and retention through porous media. Here, lab scale column experiments are used to understand the possible transport and retention of these nanoparticles.

 JoVE Chemistry

Synthetic Methodology for Asymmetric Ferrocene Derived Bio-conjugate Systems via Solid Phase Resin-based Methodology

1Department of Chemistry, Texas Christian University


JoVE 52399

The synthesis of asymmetric species of ferrocene is challenging using solution techniques. This report focuses on the methods carried out to produce a ferrocene-biotin bioconjugate using facile and clean reactions accomplished via solid-phase synthesis. Incorporation of a thiolate moiety is shown to impart the ability for immobilization on gold surfaces.

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