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JoVE Chemistry
JoVE Chemistry surveys a broad scope of chemical disciplines. This collection includes fundamental and applied research that investigates molecular interactions, properties, and structure spanning in the following core divisions: analytical, organic, organometallic, inorganic, bioinorganic, and biochemistry. This section also includes the design and preparation of materials and small molecules for advanced or unique applications.
 JoVE Chemistry

Activating Molecules, Ions, and Solid Particles with Acoustic Cavitation

1Marcoule Institute for Separative Chemistry, UMR 5257 CEA-CNRS-UM2-ENSCM


JoVE 51237

Acoustic cavitation in liquids submitted to power ultrasound creates transient extreme conditions inside the collapsing bubbles, which are the origin of unusual chemical reactivity and light emission, known as sonoluminescence. In the presence of noble gases, nonequilibrium plasma is formed. The "hot" particles and the photons generated by collapsing bubbles are able to excite species in solution.

 JoVE Chemistry

Hot Biological Catalysis: Isothermal Titration Calorimetry to Characterize Enzymatic Reactions

1Laboratory of Bioinorganic Chemistry, Department of Pharmacy and Biotechnology, University of Bologna


JoVE 51487

Isothermal titration calorimetry measures heat flow released or absorbed in chemical reactions. This method can be used to quantify enzyme-catalysis. In this paper, the protocol for instrumental setup, experiment running, and data analysis is generally described, and applied to the characterization of enzymatic urea hydrolysis by jack bean urease.

 JoVE Chemistry

Retropinacol/Cross-pinacol Coupling Reactions - A Catalytic Access to 1,2-Unsymmetrical Diols

1Department of Chemistry, Humboldt University of Berlin


JoVE 51258

A novel account for the synthesis of unsymmetrical 1,2-diols based on a retropinacol/cross-pinacol coupling mechanism is described. Due to the catalytic execution of this reaction a considerable improvement compared to conventional cross-pinacol couplings is achieved.

 JoVE Chemistry

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization

1Department of Chemistry and Biochemistry, University of Bern


JoVE 51385

The protocol described herein aims to explain and abridge the numerous obstacles in the way of the intricate route leading to modified nucleoside triphosphates. Consequently, this protocol facilitates both the synthesis of these activated building-blocks and their availability for practical applications.

 JoVE Chemistry

Thin-layer Chromatographic (TLC) Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds

1Forage-Animal Production Research Unit, Agricultural Research Service, United States Department of Agriculture


JoVE 51411

Methods are described for thin-layer chromatographic (TLC) separation of plant extracts and contact bioautography to identify antibacterial metabolites. The methods are applied to the screening of red clover phenolic compounds inhibiting hyper ammonia-producing bacteria (HAB) native to the bovine rumen.

 JoVE Chemistry

Mizoroki-Heck Cross-coupling Reactions Catalyzed by Dichloro{bis[1,1',1''-(phosphinetriyl)tripiperidine]}palladium Under Mild Reaction Conditions

1Institute of Inorganic Chemistry, University of Zürich, 2Institute of Chemistry & Biological Chemistry, Zürich University of Applied Sciences


JoVE 51444

Dichloro{bis[1,1',1''-(phosphinetriyl)tripiperidine]}palladium [(P(NC5H10)3)2Pd(Cl)2] (1) is an easy accessible, cheap, and air stable, but highly active Heck catalyst with an excellent functional group tolerance that efficiently operates under mild reaction conditions to give the coupling products in very high yields.

 JoVE Chemistry

The Use of Gas Chromatography to Analyze Compositional Changes of Fatty Acids in Rat Liver Tissue during Pregnancy

1Human Development & Health Academic Unit, Faculty of Medicine, University of Southampton


JoVE 51445

Pregnancy leads to significant changes to the fatty acid composition of maternal tissues. Lipid profiles can be obtained via gas chromatography to allow identification and quantification of fatty acids in individual lipid classes among rats fed various high and low fat diets during pregnancy.

 JoVE Chemistry

Manufacturing of Three-dimensionally Microstructured Nanocomposites through Microfluidic Infiltration

1Center for Applied Research on Polymers and Composites (CREPEC), Mechanical Engineering Department, École Polytechnique de Montréal


JoVE 51512

Three-dimensional (3D) microstructured composite beams are fabricated through the directed and localized infiltration of nanocomposites into 3D porous microfluidic networks. The flexibility of this manufacturing method enables the utilization of different thermosetting materials and nanofillers in order to achieve a variety of functional 3D reinforced nanocomposite macroscopic products.

 JoVE Chemistry

Preparing Silica Aerogel Monoliths via a Rapid Supercritical Extraction Method

1Department of Chemistry, Union College, 2Department of Mechanical Engineering, Union College


JoVE 51421

This article describes a rapid supercritical extraction method for fabricating silica aerogels. By utilizing a confined mold and hydraulic hot press, monolithic aerogels can be made in eight hours or less.

 JoVE Chemistry

Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects

1Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University


JoVE 51383

The protocols here describe kinetic assays of protein-protein interactions with Bio-layer Interferometry. F-type ATP synthase, which is involved in cellular energy metabolism, can be inhibited by its ε subunit in bacteria. We have adapted Bio-layer Interferometry to study interactions of the catalytic complex with ε’s inhibitory C-terminal domain.

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