Overview
In proton NMR spectroscopy, primary amines and secondary amines showcase their N–H protons as a broad signal in the chemical shift range between δ 0.5 and 5 ppm. The exact position in this range depends on several factors, including sample concentration, hydrogen bonding, and the type of solvent used. Since amine protons undergo fast proton exchange in solution, the protons are labile and therefore do not participate in any splitting with adjacent protons. Thus, the observed peak is broadd does not provide any information about the adjacent proton environment. This problem is resolved, and N–H protons are made identifiable, by adding D2O to the mixture. The addition causes the N–H protons to exchange with deuterons, leading to the disappearance of N–H proton peaks. This disappearance is indicative of the presence of labile protons in the sample.…
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