Summary
This protocol details how to quantify synapse number both in dissociated neuronal culture and in brain sections using immunocytochemistry. Using compartment-specific antibodies, we label presynaptic terminals as well as sites of postsynaptic specialization. We define synapses as points of colocalization between the signals generated by these markers.
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Embed code vs URL
An embed code allows you to place a JoVE video directly inside of your learning or training environment. Most learning management systems such as Canvas, Blackboard, and Moodle require an embed code for JoVE content to stream.
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Most of the time, you will want to use the embed code. However, there are some environments that don’t support embed codes such as Google Classrooms and MS Teams. In those instances, you can use a URL to still provide access to JoVE videos.
What is an Embed Code?
Basically, it’s a snippet of code that you put on your website that displays as a video. An embed code pulls the video from the original source, allowing you to display a video without having to host the file on your website.
How to embed a video
STEP 1: COPY YOUR EMBED CODE
To generate the embed code, simply click the Copy button above. You can customize it by choosing a specific language for the video, or setting exact dimensions for the video player.
STEP 2: PASTE THE EMBED CODE INTO YOUR ENVIRONMENT
Embed codes can be used in most learning management systems (Canvas, Blackboard, Moodle…), organizational websites, and a host of other tools. The specifics of how to add in an embed code will depend on your environment.
Generally, it involves accessing a text editor, entering the HTML mode on the editor, and pasting in the embed code. Get more details on embedding into specific environments here.