Journal of Visualized Experiments

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Keyword Search: Plant Biology

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Video Articles

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Keywords
A rapid way is described to gain insights into the structure of polysaccharides in an extracellular matrix. The method takes advantage of the specificity of glycosylhydrolases and the sensitivity of mass spectrometry allowing minute amounts of materials to be analyzed. This technique is adaptable to be used directly on tissue itself.
Launch Video

    Published: 06/20/2010
Markus Günl1
Sascha Gille1
Markus Pauly1, 2

1Energy Biosciences Institute, University of California, Berkeley

2Department of Plant and Microbial Biology, University of California, Berkeley

 
This article illustrates how to properly use the BioRad Helios Gene Gun to introduce plasmid DNA into onion epidermal cells and how to test for protein-protein interactions in onion cells based on the principle of Bimolecular Fluorescence Complementation (BiFC)
Launch Video

    Published: 06/12/2010
Courtney A. Hollender
Zhongchi Liu

Dept. Of Cell Biology and Molecular Genetics, University of Maryland

 
The molecular basis of spatial-specific phytochrome responses is being investigated using transgenic plants that exhibit tissue- and organ-specific phytochrome deficiencies. The isolation of specific cells exhibiting induced phytochrome chromophore depletion by Fluorescence-Activated Cell Sorting followed by microarray analyses is being utilized to identify genes involved in spatial-specific phytochrome responses.
Launch Video

    Published: 05/29/2010
Sankalpi N. Warnasooriya1
Beronda L. Montgomery1, 2

1Department of Energy - Plant Research Laboratory, Michigan State University

2Department of Biochemistry and Molecular Biology, Michigan State University

 
We report a method for introduction, tracking and quantitative analysis of GFP expression in plant cells. This method utilizes a custom-designed robotics system for semi-continuous image collection from large numbers of samples, over time. We also demonstrate the use of ImageJ and ImageReady for analysis of image series.
Launch Video

2 Comments    Published: 05/05/2010
Carlos M. Hernandez-Garcia1
Joseph M. Chiera1, 2
John J. Finer1

1Department of Horticulture and Crop Science, OARDC/The Ohio State University

2Department of Plant Pathology, North Carolina State University

 
Tomato Analyzer (TA) quantifies attributes of two dimensional shapes and color in a reproducible and accurate manner. A step-by-step procedure for obtaining high quality digitalized images of tomato fruit, morphological and color analyses of these images and several applications using the data generated through this software are described.
Launch Video

2 Comments    Published: 03/16/2010
Gustavo R. Rodríguez
Jennifer B. Moyseenko
Matthew D. Robbins
Nancy Huarachi Morejón
David M. Francis
Esther van der Knaap

Department of Horticulture and Crop Science, The Ohio State University/OARDC

 
Plant biomass is a major carbon-neutral renewable resource that could be used for the production of biofuels. Plant biomass consists mainly of cell walls, a structurally complex composite material termed lignocellulosics. Here we describe a protocol for a comprehensive analysis of the content and composition of wall derived carbohydrates.
Launch Video

    Published: 03/12/2010
Cliff E. Foster1
Tina M. Martin1
Markus Pauly2

1Great Lakes Bioenergy Research Center, Michigan State University

2Great Lakes Bioenergy Research Center and DOE-Plant Research Lab, Michigan State University

 
Plant biomass is a major carbon-neutral renewable resource that could be used for the production of biofuels. Plant biomass consists mainly of cell walls, a structurally complex composite material termed lignocellulosics. Here we describe a protocol for a comprehensive analysis of the content and composition of the polyphenolic lignin.
Launch Video

2 Comments    Published: 03/11/2010
Cliff E. Foster1
Tina M. Martin1
Markus Pauly2

1Great Lakes Bioenergy Research Center, Michigan State University

2Great Lakes Bioenergy Research Center and DOE-Plant Research Lab, Michigan State University

 
Many plant tissues, including phloem and xylem from loblolly pine (Pinus taeda L.), contain high levels of phenolics and polysaccharides that interfere with RNA purification. This presentation discusses techniques for the harvest of field-grown tissues and isolation of RNA of sufficient quality for microarrays and other genomic analyses.
Launch Video

    Published: 02/22/2010
W. Walter Lorenz
Yuan-Sheng Yu
Jeffrey F. D. Dean

Warnell School of Forestry and Natural Resources, University of Georgia

 
A method for isolating specific cell types from plant material is demonstrated. This technique employs transgenic marker lines expressing fluorescent proteins in particular cell types, cellular dissociation and Fluorescence Activated Cell Sorting. Additionally, a growth setup is established here that facilitates treatment of Arabidopsis thaliana seedlings prior to cell sorting.
Launch Video

2 Comments    Published: 02/18/2010
Bastiaan O. R. Bargmann
Kenneth D. Birnbaum

Center for Genomics and Systems Biology, Department of Biology, New York University

 
A cell death-based assay for PTI in Nicotiana benthamiana plants is described.
Launch Video

    Published: 09/09/2009
Suma Chakravarthy1
André C. Velásquez1, 2
Gregory B. Martin1, 2

1Boyce Thompson Institute for Plant Research

2Plant Pathology and Plant-Microbe Biology, Cornell University

 

 

Page:1 2 3 

 

Total: 24 articles.

 

Search results for 7/31/2010 4:10:55 PM

 

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Journal of Visualized Experiments (JoVE) is an online research journal employing visualization to increase reproducibility and transparency in biological sciences.

 

ISSN 1940-087X

 

 

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