Reproductive Medical Centre of Guangdong Women and Children's Hospital 1 article published in JoVE Developmental Biology Use of a Recombinant Mosquito Densovirus As a Gene Delivery Vector for the Functional Analysis of Genes in Mosquito Larvae Pei-Wen Liu1, Jia-Bao Xu1, Yun-Qiao Dong2, Xiao-Guang Chen1, Jin-Bao Gu1 1Department of Pathogen Biology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, 2 We report using an artificial intronic small RNA expression strategy to develop a non-defective recombinant Aedes aegypti densovirus (AaeDV) in vivo delivery system. A detailed procedure for the construction, packaging, and quantitative analysis of the rAaeDV vectors as well as for larval infection is described.
