Jiangsu Academy of Agricultural Sciences 2 articles published in JoVE Environment Specific and Accurate Detection of the Citrus Greening Pathogen Candidatus liberibacter spp. Using Conventional PCR on Citrus Leaf Tissue Samples Huan Chen*1,2, Ian Arthur Palmer*1, Jian Chen1,2, Ming Chang1,2, Stephen L. Thompson3, Fengquan Liu2, Zheng Qing Fu1 1Department of Biological Sciences, University of South Carolina, 2Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, 3Department of Instructions and Teacher Education, University of South Carolina Citrus Greening is a particularly destructive disease affecting citrus crops globally. Presented here is a simple method using PCR and genomic DNA extraction of citrus leaf tissue for the accurate and precise identification of the citrus greening pathogen, Candidatus liberibacter spp. Biology Measuring Spatial and Temporal Ca2+ Signals in Arabidopsis Plants Xiaohong Zhu1, Aaron Taylor2, Shenyu Zhang1, Dayong Zhang1,3, Ying Feng1,4, Gaimei Liang1,5, Jian-Kang Zhu1,6 1Department of Horticulture and Landscape Architecture, Purdue University, 2Bindley Bioscience Center, Purdue University, 3Institute of Biotechnology, Jiangsu Academy of Agricultural Sciences, 4College of Environmental & Resource Science, Zhejiang University, 5Dryland Agriculture Research Centre, Shanxi Academy of Agricultural Sciences, 6Shanghai Center for Plant Stress Biology, Chinese Academy of Sciences Ca2+ signaling regulates diverse biological processes in plants. Here we present approaches for monitoring abiotic stress induced spatial and temporal Ca2+ signals in Arabidopsis cells and tissues using the genetically encoded Ca2+ indicators Aequorin or Case12.
