Nanchang University 1 article published in JoVE Neuroscience The Cutting and Floating Method for Paraffin-embedded Tissue for Sectioning Cheng Qin*1,2, Yijiang Bai*1,2, Zhen Zeng1,2, Liao Wang1,2, Zhiwen Luo1,2, Shunqi Wang1,3, Suqi Zou1,3 1Institute of Life Science, Nanchang University, 2Queen Mary School, Medical Department, Nanchang University, 3School of Life Science, Nanchang University Here, we present a protocol to improve paraffin sectioning. This method combines cutting and floating using a simple thermostatic chamber to avoid the transfer process required by the conventional method. As a result, the efficiency and the number of intact paraffin sections were greatly improved.