Shantou University Medical College 7 articles published in JoVE Medicine A Two-Step Method for Percutaneous Transhepatic Choledochoscopic Lithotomy Zeming Chen1, Zhuo Hua1, Runzhui Lin1, Hongcai Zhuang1, Xingmu Liu1 1Department of General Surgery, Second Affiliated Hospital, Shantou University Medical College This "two-step method" significantly improved the success rate of percutaneous transhepatic choledochoscopy and achieved a better prognosis of intrahepatic and extrahepatic choledocholithiasis. Medicine Application of Optical Coherence Tomography to a Mouse Model of Retinopathy Xiaoting Mai*1, Shaofen Huang*1, Wen Chen1, Tsz Kin Ng1,2,3, Haoyu Chen1 1Joint Shantou International Eye Center, Shantou University and the Chinese University of Hong Kong, 2Shantou University Medical College, 3Department of Ophthalmology and Visual Sciences, the Chinese University of Hong Kong Here, we describe an in vivo imaging technique using optical coherence tomography to facilitate the diagnosis and quantitative measurement of retinopathy in mice. Immunology and Infection An Efficient Method for Directed Hepatocyte-Like Cell Induction from Human Embryonic Stem Cells Qi Zhou*1,2,3, Xiaoling Xie*1,2,3, Zhiqian Zhong1,2,3, Pingnan Sun1,2,3, Xiaoling Zhou1,2,3 1Stem Cell Research Center, Shantou University Medical College, 2The Center for Reproductive Medicine, Shantou University Medical College, 3Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology, Shantou University Medical College This manuscript describes a detailed protocol for differentiation of human embryonic stem cells (hESCs) into functional hepatocyte-like cells (HLCs) by continuously supplementing Activin A and CHIR99021 during hESC differentiation into definitive endoderm (DE). Immunology and Infection Modeling Hepatitis B Virus Infection in Non-Hepatic 293T-NE-3NRs Cells Xiaoyue Sun*1,2,3, Xiaoqiang Yang*1,2,3,4, Chui Zeng1,2,3, Fayed Attia Koutb Megahed1,2,3,5, Qi Zhou1,2,3, Tingdang Liu1,2,3, Waqas Iqbal1,2,3, Pingnan Sun1,2,3, Xiaoling Zhou1,2,3 1Stem Cell Research Center, Shantou University Medical College, 2The Center for Reproductive Medicine, Shantou University Medical College, 3Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology, Shantou University Medical College, 4Medical Research Center, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, 5Department of Nucleic Acid Researches, Genetic Engineering and Biotechnology Research Institute, General Autority - City of Scientific Researches and Technological Applications This manuscript describes a detailed protocol for Hepatitis B virus (HBV) infection in novel engineered 293T cells (293T-NE-3NRs, expressing human NTCP, HNF4α, RXRα and PPARα) and traditional hepatic cells (HepG2-NE, expressing human NTCP). Cancer Research Performing Data Mining And Integrative Analysis Of Biomarker in Breast Cancer Using Multiple Publicly Accessible Databases Min-na Chen1, De Zeng2, Zhuo-qun Zheng3, Zheng Li3, Jian-le Wu3, Jun-yu Jin3, He-jia Wang3, Cui-zhen Huang1, Hao-yu Lin1 1Department of Thyroid and Breast Surgery, First Affiliated Hospital of Shantou University Medical College, 2Department of Medical Oncology, Cancer Hospital of Shantou University Medical College, 3Shantou University Medical College Here, we present a protocol to explore the biomarker and survival predictor of breast cancer based on the comprehensive analysis of pooled clinical datasets derived from a variety of publicly accessible databases, using the strategy of expression, correlation and survival analysis step by step. Medicine Preparation, Procedures and Evaluation of Platelet-Rich Plasma Injection in the Treatment of Knee Osteoarthritis Ziming Chen*1,2, Zhantao Deng*1, Yuanchen Ma1, Junxing Liao1, Qingtian Li1, Mengyuan Li1, Hua Liu1, Ganghong Chen1, Chaoming Zeng1, Qiujian Zheng1 1Department of Orthopedics, Guangdong General Hospital (Guangdong Academy of Medical Sciences), 2Shantou University Medical College Knee osteoarthritis is frequently seen in the orthopedic department. We introduce in detail the entire knee osteoarthritis treatment process with platelet-rich plasma injection, including preparation, procedures, and evaluation. Developmental Biology A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation In Vitro Zhaofeng Jia1,2, Yujie Liang3, Bin Ma4,5, Xiao Xu2,6, Jianyi Xiong2, Li Duan2, Daping Wang1,2 1Guangzhou Medical University, 2 We present a method to quantify DNA methylation based on the 5-methylcytosine (5-mC) dot blot. We determined the 5-mC levels during chondrocyte dedifferentiation. This simple technique could be used to quickly determine the chondrocyte phenotype in ACI treatment.
