Fujian Key Laboratory of Organ and Tissue Regeneration 1 article published in JoVE Immunology and Infection Optimization of the Cuff Technique for Murine Heart Transplantation Yunhan Ma*1,2, Baiyi Xie*1,2, Helong Dai*3,4,5, Chenxi Wang6, Shujiao Liu6, Tianshu Lan7, Shuangyue Xu1,2, Guoliang Yan1,2,6, Zhongquan Qi1,2,8 1Organ Transplantation Institute, School of Medicine, Xiamen University, 2Fujian Key Laboratory of Organ and Tissue Regeneration, 3Department of Kidney Transplantation, Center of Organ Transplantation, The Second Xiangya Hospital of Central South University, 4Clinical Research Center for Organ Transplantation in Hunan Province, 5Clinical Immunology Center, Central South University, 6School of Medicine, Xiamen University, 7Xiamen Medical College, 8Medical College, Guangxi University We introduce an inner tube approach to the cuff technique for mouse cervical heterotopic heart transplantation to help evert the vessel over the cuff. We found that cooperation between two experienced surgeons markedly shortens the operation time.
