Liver-biotechnology (Shenzhen) Co., Ltd. 1 article published in JoVE Biology Quantification of Circulating Pig-Specific DNA in the Blood of a Xenotransplantation Model Yangyang Deng*1,2, Ming Zhou*1,3, Ying Lu1, Jiao Chen1, Zuhui Pu4, Dongjing Yu1,5, Yifan Dai6, Yongqiang Zhan7, Lisha Mou1 1 In this protocol, porcine specific primers were designed, plasmids-containing porcine specific DNA fragments were constructed, and standard curves for quantitation were established. Using species-specific primers, cpsDNA was quantified by qPCR in pig-to-mouse cell transplantation models and pig-to-monkey artery patch transplantation models.
