Shenzhen Second People's Hospital/First Affiliated Hospital of Shenzhen University 2 articles published in JoVE Biology Large-Scale Preparation of Synovial Fluid Mesenchymal Stem Cell-Derived Exosomes by 3D Bioreactor Culture Li Duan1,2, Xingfu Li1,2, Xiao Xu1,2, Limei Xu1,2, Daping Wang1,2, Kan Ouyang1,2, Yujie Liang1,3 1Guangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology, Shenzhen Second People's Hospital, 3Department of Child and Adolescent Psychiatry, Shenzhen Kangning Hospital, Shenzhen Mental Health Center, Shenzhen Key Laboratory for Psychological Healthcare & Shenzhen Institute of Mental Health Here, we present a protocol to produce a large number of GMP-grade exosomes from synovial fluid mesenchymal stem cells using a 3D bioreactor. Biology Quantification of Circulating Pig-Specific DNA in the Blood of a Xenotransplantation Model Yangyang Deng*1,2, Ming Zhou*1,3, Ying Lu1, Jiao Chen1, Zuhui Pu4, Dongjing Yu1,5, Yifan Dai6, Yongqiang Zhan7, Lisha Mou1 1 In this protocol, porcine specific primers were designed, plasmids-containing porcine specific DNA fragments were constructed, and standard curves for quantitation were established. Using species-specific primers, cpsDNA was quantified by qPCR in pig-to-mouse cell transplantation models and pig-to-monkey artery patch transplantation models.
