Guangzhou Insititute of Oral Disease 1 article published in JoVE Bioengineering Propagation of Dental and Respiratory Cells and Organs in Microgravity Mirali Pandya1, Wei Ma1,2, Huling Lyu1,3, Xianghong Luan1, Thomas G. H. Diekwisch1 1Center for Craniofacial Research and Diagnosis, Texas A&M College of Dentistry, 2Department of Stomatology, The Fourth Affiliated Hospital, Harbin Medical University, 3Key Laboratory of Oral Medicine, Guangzhou Insititute of Oral Disease, Stomatology Hospital of Guangzhou Medical University This protocol presents a method for the culture and 3D growth of ameloblast-like cells in microgravity to maintain their elongated and polarized shape as well as enamel-specific protein expression. Culture conditions for the culture of periodontal engineering constructs and lung organs in microgravity are also described.
