Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center 1 article published in JoVE Biochemistry Purification of Endogenous Drosophila Transient Receptor Potential Channels Jia Liu*1, Yuyang Liu*1, Weidi Chen1, Yuzhen Ding1, Xiaoru Lan1, Wei Liu1 1Shenzhen Key Laboratory for Neuronal Structural Biology, Biomedical Research Institute, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center Based on the assembling mechanism of the INAD protein complex, in this protocol, a modified affinity purification plus competition strategy was developed to purify the endogenous Drosophila TRP channel.
