Key Laboratory of Acupuncture and Medicine Research of Ministry of Education 1 article published in JoVE Medicine Improved Rodent Model of Myocardial Ischemia and Reperfusion Injury Hua-Qin Tong*1,2,3, Man-Lu Fan*3, Tong Sun3, Hao-Wen Zhang4, Jie Han5, Meng-Xi Wang3, Jian-Dong Chen5, Wei-Xin Sun6, Xiao-Hu Chen1,2, Mian-Hua Wu7 1Department of Cardiology, Affiliated Hospital of Nanjing University of Chinese Medicine, 2Department of Cardiology, Jiangsu Province Hospital of Chinese Medicine, 3First College of Clinical Medicine, Biological Technology Center for Innovation in Chinese Medicine, Nanjing University of Chinese Medicine, 4School of Health Preservation and Rehabilitation, Key Laboratory of Acupuncture and Medicine Research of Ministry of Education, 5Department of Cardiology, Jiangsu Provincial Hospital of Chinese Medicine, 6Department of Cardiology, Yancheng TCM Hospital Affiliated to Nanjing University of Chinese Medicine, 7Jiangsu Collaborative Innovation Center of Traditional Chinese Medicine (TCM) Prevention and Treatment of Tumor, Nanjing University of Chinese Medicine Myocardial ischemia-reperfusion model of rat heart is improved by using a self-made retractor, polyvinyl chloride tube, and a unique knotting method. Electrocardiogram, triphenyltetrazolium chloride and histological staining, and percent survival analysis results showed that the improved model group has higher success and survival rates than the already existing model group.
