Chinese Academy of Medical Sciences 3 articles published in JoVE Immunology and Infection Standardization of Transfer across Labs between Flow Cytometers for Detection of Lymphocytes in Japanese Encephalitis Vaccinated Children Huizheng Ge*1, Linlin Zhang*2,3, Mengjia Liu*2,3, Xi Yang1, Xu Wu1, Ran Wang2,3, Zhengde Xie2,3 1Center of Excellence (Beijing), Becton Dickinson Medical Devices (Shanghai) Co Ltd, 2 In this study, a method was developed to facilitate the transfer of experimental settings and analysis templates between two flow cytometers in two laboratories for the detection of lymphocytes in Japanese encephalitis-vaccinated children. The standardization method for the flow cytometer experiments will allow research projects to be effectively conducted in multiple centers. Immunology and Infection Detection of Polyfunctional T Cells in Children Vaccinated with Japanese Encephalitis Vaccine via the Flow Cytometry Technique Linlin Zhang*1,2, Meng Zhang*1,2,3, Mengjia Liu1,2, Junhong Ai1,2, Jiao Tian1,2, Huizheng Ge4, Ran Wang1,2, Zhengde Xie1,2 1 The present protocol combines ex vivo stimulation and flow cytometry to analyze polyfunctional T cell (TPF) profiles in peripheral blood mononuclear cells (PBMCs) within Japanese encephalitis virus (JEV)-vaccinated children. The detection method and flow cytometry color scheme of JEV-specific TPFs were tested to provide a reference for similar studies. Immunology and Infection Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis Linlin Zhang1,2, Mengjia Liu1,2, Meng Zhang1,2, Junhong Ai1,2, Jiao Tian1,2, Ran Wang1,2, Zhengde Xie1,2 1 We describe a method combining immunomagnetic beads and fluorescence-activated cell sorting to isolate and analyze defined immune cell subpopulations of peripheral blood mononuclear cells (monocytes, CD4+ T cells, CD8+ T cells, B cells, and natural killer cells). Using this method, magnetic and fluorescently labeled cells can be purified and analyzed.
