Third Military Medical University of Chinese PLA 2 articles published in JoVE Immunology and Infection In Vitro Cellular Activity Evaluation of the Nanoemulsion Vaccine Adjuvant Ophiopogonin D Xing Luo1, Yanan Tong1, Xiaoqiang Zeng1, Yan Ye1, Yun Yang1, Zhen Song1, Zelong Zhang1, Haibo Li1, Jining Gao2, Xuhu Mao1, Hao Zeng1, Quanming Zou1, Hongwu Sun1 1Department of Microbiology and Biochemical Pharmacy, National Engineering Research Centre of Immunological Products, College of Pharmacy, Third Military Medical University of Chinese PLA, 2Institute of Combined Injury of PLA, College of Military Preventive Medicine, Third Military Medical University of Chinese PLA The protocol presents detailed methods for evaluating whether the nanoemulsion ophiopogonin D adjuvant promotes effective cellular immune responses. Immunology and Infection Preparation, Characteristics, Toxicity, and Efficacy Evaluation of the Nasal Self-Assembled Nanoemulsion Tumor Vaccine In Vitro and In Vivo Zelong Zhang1, Dingyi Cai1, Shuang Ge1, Xing Luo1, Xiaoqiang Zeng1, Yan Ye1, Zhen Song1, Liusheng Peng1, Haibo Li1, Quanming Zou1, Hao Zeng1, Hongwu Sun1, Yun Yang1 1National Engineering Research Centre of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical University of Chinese PLA Here, we present detailed methods for the preparation and evaluation of the nasal self-assembled nanoemulsion tumor vaccine in vitro and in vivo.
