Hebei University 2 articles published in JoVE Biology Optimizing the Rearing Procedure of Germ-Free Wasps Zhengyu Zhu*1,2, Dandan Wang*1, Yanjun Liu1, Ting Tang2, Guan-Hong Wang1 1State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, 2College of Life Sciences, Hebei University Nasonia wasp embryos were dissected from Lucillia sericata pupae after parasitization for 12-24 h and washed with alcohol and 10% sodium hypochlorite solution to obtain germ-free embryos. After rearing the germ-free embryos and supplying them with Nasonia rearing medium to grow and develop in vitro, germ-free Nasonia adults were obtained. Biochemistry Identification of Peptides of Small Extracellular Vesicles from Bone Marrow-Derived Macrophages Jiale Cheng1,2, Jiong Zhu1,2, Yujiao Liu2,3, Chen Yang2,3, Yuehui Zhang2, Yuchen Liu2, Chaozhi Jin2, Jian Wang1,2 1School of Basic Medical Sciences, Anhui Medical University, 2State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, 3School of Life Sciences, Hebei University This protocol describes a procedure to isolate small extracellular vesicles from macrophages by differential ultracentrifugation and extract the peptidome for identification by mass spectrometry.