Beijing Institute of Lifeomics 1 article published in JoVE Biochemistry Identification of Peptides of Small Extracellular Vesicles from Bone Marrow-Derived Macrophages Jiale Cheng1,2, Jiong Zhu1,2, Yujiao Liu2,3, Chen Yang2,3, Yuehui Zhang2, Yuchen Liu2, Chaozhi Jin2, Jian Wang1,2 1School of Basic Medical Sciences, Anhui Medical University, 2State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, 3School of Life Sciences, Hebei University This protocol describes a procedure to isolate small extracellular vesicles from macrophages by differential ultracentrifugation and extract the peptidome for identification by mass spectrometry.
